Cuminum cyminum Ameliorates Urotoxic Effects of Cyclophosphamide by Modulating Antioxidant, Inflammatory Cytokines, and Urinary Bladder Overactivity: In vivo and In Silioc investigations

by Selleckchem | Dec 02 2023

This study aimed to investigate the effect of aqueous ethanol extract of Cuminum cyminum (AEECC) on oxidative stress, inflammation and overactivity of the urinary bladder induced by cyclophosphamide (CYP). The enhanced nociception behavior, bladder weight, vascular permeability, edema, hemorrhage, nitric oxide, IL-6 and TNF-α levels by CYP administration were significantly reduced by AEECC (250 and 500 mg/kg). A significant increase in serum antioxidant systems such as CAT and GPx was also observed in AEECC-treated rats. The AEECC (3 mg/ml) significantly reduced urinary bladder tone in the strips pre-contracted with carbachol in both control and CYP-treated rats. This relaxation was demolished by atropine, nifedipine, glibenclamide, and indomethacin but not with propranolol. The plant extract showed the presence of antioxidant and anti-inflammatory phytochemicals. These results suggest that Cuminum cyminum offers uroprotective activity and can ameliorate CYP-induced bladder toxicity by modulating antioxidant parameters, pro-inflammatory cytokine levels and bladder smooth muscle overactivity. The in silico binding interactions of anti-oxidant 2I3Y and anti-inflammatory protein 1TNF with various ligands from Cuminum cyminum seeds reveal potential bioactive compounds with promising anti-oxidant and anti-inflammatory properties, providing valuable insights for drug development and nutraceutical research.

Comments:

The study you provided explores the effects of the aqueous ethanol extract of Cuminum cyminum (AEECC) on oxidative stress, inflammation, and overactivity of the urinary bladder induced by cyclophosphamide (CYP) in rats. The results indicate that AEECC significantly reduced various indicators of bladder damage and inflammation caused by CYP administration. Here's a breakdown of the findings:

1. **Reduction in Nociception and Bladder Changes**: AEECC reduced enhanced nociception behavior, bladder weight, vascular permeability, edema, and hemorrhage caused by CYP.

2. **Reduction in Inflammatory Markers**: AEECC reduced elevated levels of nitric oxide, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) induced by CYP administration, indicating its anti-inflammatory effects.

3. **Enhanced Antioxidant Systems**: AEECC increased the activity of serum antioxidant enzymes such as catalase (CAT) and glutathione peroxidase (GPx), suggesting its ability to modulate oxidative stress.

4. **Bladder Relaxation**: AEECC caused a significant reduction in urinary bladder tone, particularly in the presence of carbachol, and this effect was blocked by specific inhibitors, indicating the involvement of specific pathways in this relaxation response.

5. **In Silico Analysis**: The study also conducted in silico (computer-based) analyses of the interactions between proteins associated with antioxidant and anti-inflammatory properties and various compounds from Cuminum cyminum seeds. This analysis identified potential bioactive compounds with antioxidant and anti-inflammatory properties, suggesting the medicinal potential of these compounds for drug development and nutraceutical research.

In summary, the findings of this study suggest that Cuminum cyminum has uroprotective activity. It can ameliorate bladder toxicity induced by cyclophosphamide through its antioxidant properties, modulation of pro-inflammatory cytokine levels, and regulation of bladder smooth muscle overactivity. Furthermore, the study's in silico analysis provides valuable insights into potential bioactive compounds present in Cuminum cyminum seeds, which could be explored for therapeutic purposes in the future.