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2x SYBR Green qPCR Master Mix (Low ROX)

For research use only.

Selleck's has been cited by 93 publications

Advantages

High Sensitivity: Lower Ct values ensure higher efficiency of amplification

Cost Effective: Offered at much discounted price compared to market price

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Description

2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with low concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 7500, 7500 Fast, ViiA^TM7; Stratagene MX4000^TM, MX3005P^TM, MX3000P^TM.

Applications

Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications

Components

Component	Cat#: B21702	Cat#: B21703
2x SYBR Green qPCR Master Mix(Low ROX) *	5 mL (for 200 reactions with 50 μl/rxn for 500 reactions with 20 μl/rxn for 1000 reactions with 10 μl/rxn)	25 mL (for 1000 reactions with 50 μl/rxn for 2500 reactions with 20 μl/rxn for 5000 reactions with 10 μl/rxn)
* Contains hot-start DNA Polymerase, dNTPs, Mg²⁺, and SYBR Green I dye and ROX Dye (Low concentration)

Storage (From the date of receipt)

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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