2x SYBR Green qPCR Master Mix

Selleck's Has Been Cited by 848 Publications

Advantages

High Sensitivity: Lower Ct values ensure higher efficiency of amplification

Cost Effective: Offered at much discounted price compared to market price

Product Comparison

Higher amplification efficiency and stronger fluorescence signal

Selleck 2x SYBR Green qPCR Master Mix provides higher amplification efficiency and brighter fluorescence signal

Using the same cDNA template and primers to amplify GAPDH, Selleck 2x SYBR Green QPCR Master Mix amplified faster with stronger fluorescence signal and better amplification efficiency than other brands.

Accurate quantitation and high stability

Selleck 2x SYBR Green QPCR Master Mix shows accurate quantitation with high stability

Using the same cDNA template and primers to amplify RPL37, Selleck 2x SYBR Green QPCR Master Mix shows accurate quantitation with high stability.

Higher specificity and sensitivity

Selleck 2x SYBR Green QPCR Master Mix reduces non-specific amplification at low temperature, improving amplification of low-copy genes.

Failure to amplify low-copy targets can be due to lack of sensitivity and/or specificity. Selleck 2x SYBR Green QPCR Master Mix uses a new type of hot-start enzyme which reduces non-specific amplification at low temperature, improving amplification of low-copy genes.

For example
Ct ValueGene	Selleck	Vazyme	Yeasen	Kapa	Takara	TIANGEN	NEB	TransGen
GAPDH (Human)	19.01	20.34	18.84	19.35	19.32	19.08	19.54	19.9
Actin (Human)	18.5	20.07	19	18.71	19.9	19.4	23.64	19.86
Vim (Human)	18.55	20.19	18.56	18.82	19.9	19.11	19.27	19.83
IL-6 (Human, low Copy)	29.03	29.55	32.54	27.56	35.12	33.31	33.02	29.54
GAPDH (Mouse)	20.2	21.37	20.39	21.34	20.83	20.67	21.01	21.85
GAPDH (Mouse)	20.2	21.37	20.39	21.34	20.83	20.67	21.01	21.85
RPL37 (Mouse)	18.76	19.69	18.77	18.77	19.3	19.14	19.3	19.86

Comparison of Selleck 2x SYBR Green qPCR Master Mix-The number of the lowest Ct value

Experimental comparison in HeLa cells and the actin gene with Selleck 2x SYBR Green qPCR Master Mix

Experimental comparison in HeLa cells and the VIM gene with Selleck 2x SYBR Green qPCR Master Mix

Experimental comparison in L929 cells and the GAPDH gene with Selleck 2x SYBR Green qPCR Master Mix

Price Comparison

Description

2x SYBR Green qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

Applications

Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications

Components

Component	Cat#: B21202	Cat#: B21203
2x SYBR Green qPCR Master Mix *	5 mL (for 200 reactions with 50 μl/rxn for 500 reactions with 20 μl/rxn for 1000 reactions with 10 μl/rxn)	25 mL (for 1000 reactions with 50 μl/rxn for 2500 reactions with 20 μl/rxn for 5000 reactions with 10 μl/rxn)
50x ROX Dye (low conc.)	200 μL	1000 μL
50x ROX Dye (high conc.)	200 μL	1000 μL
* Contains hot-start DNA Polymerase, dNTPs, Mg²⁺, and SYBR Green I dye

ROX Reference Dye Selection Guide for Different Real-time PCR Instruments

Instruments Requiring No ROX Reference Dye	Bio-Rad: CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, Opticon®, Opticon 2, Chromo4™, MiniOpticon™; Cepheid: SmartCycler®; Eppendorf: Mastercycler® eprealplex, realplex 2s; Illumina: Eco™ qPCR; Qiagen/Corbett: Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche: LightCycler® 480, 96, Nano, 1.5/2.0**； Thermo Scientific: PikoReal Cycler
Instruments Requiring ROX Reference Dye 1 (High Concentration)	Applied Biosystems: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; Applied Biosystems: StepOne™, StepOne Plus™
Instruments Requiring ROX Reference Dye 2 (Low Concentration)	Applied Biosystems: 7500, 7500 Fast, Viia™7, QuantStudio 6 and 7 Flex System, QuantStudio 3 and 5; Agilent Stratagene: MX4000™, MX3005P™, MX3000P™

Storage (From the date of receipt)

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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