Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency

Microcell-mediated chromosome transfer is an attractive technique for transferring chromosomes from donor cells to recipient cells and has enabled the generation of cell lines and humanized animal models that contain megabase-sized gene(s). However, improvements in chromosomal transfer efficiency are still needed to accelerate the production of these cells and animals. The chromosomal transfer protocol consists of micronucleation, microcell formation, and fusion of donor cells with recipient cells. We found that the combination of Taxol (paclitaxel) and reversine rather than the conventional reagent colcemid resulted in highly efficient micronucleation and substantially improved chromosomal transfer efficiency from Chinese hamster ovary donor cells to HT1080 and NIH3T3 recipient cells by up to 18.3- and 4.9-fold, respectively. Furthermore, chromosome transfer efficiency to human induced pluripotent stem cells, which rarely occurred with colcemid, was also clearly improved after Taxol and reversine treatment. These results might be related to Taxol increasing the number of spindle poles, leading to multinucleation and delaying mitosis, and reversine inducing mitotic slippage and decreasing the duration of mitosis. Here, we demonstrated that an alternative optimized protocol improved chromosome transfer efficiency into various cell lines. These data advance chromosomal engineering technology and the use of human artificial chromosomes in genetic and regenerative medical research.

 

Comments:

That's an intriguing advancement in chromosomal engineering! The combination of Taxol and reversine seems to have significantly enhanced the efficiency of microcell-mediated chromosome transfer. Taxol's role in increasing spindle poles and inducing multinucleation, along with reversine's impact on mitotic slippage and shortened mitosis duration, appears to be the key factors behind this improvement.

This advancement holds promise for various applications, including the generation of cell lines, humanized animal models, and specifically, the more efficient transfer of chromosomes into human induced pluripotent stem cells. The increased efficiency in chromosome transfer is critical for advancing genetic and regenerative medical research using human artificial chromosomes.

It's fascinating how optimizing the protocol can have such a significant impact on the efficiency of chromosome transfer across different cell lines. This kind of advancement has the potential to accelerate research in genetics and regenerative medicine, potentially opening up new avenues for exploring gene therapies and understanding complex genetic diseases.

Related Products

Cat.No.	Product Name	Information
E2631	Colcemid	Colcemid (Demecolcine), a derivative of colchicine, is a potent mitotic inhibitor which can incuces cell apoptosis and can be used for cancer research.

Related Targets

Apoptosis related