Three new phenolic glycosides and a new lignan glycoside from Gaultheria leucocarpa var. yunnanensis

Three new phenolic glycosides (1-3) and a new lignan glycoside (4), together with five known compounds (5-9) were isolated from the ethanol extract of the aerial part of Gaultheria leucocarpa var. yunnanensis (Franch.) T.Z.Hsu & R.C.Fang. Their structures were determined on the basis of spectroscopic techniques, experimental and calculated ECD spectra, acid hydrolysis, and enzymatic hydrolysis experiments. All the isolates were evaluated for their anti-inflammatory and antioxidant activities. Compounds 7 and 8 exhibited inhibitory effects against the LPS-induced production of NO with IC50 of 63.71 and 10.66 μM, respectively, compared to L-NMMA having an IC50 of 6.95 μM. Besides, compound 7 also represented significant DPPH radical scavenging activity with EC50 of 18.75 μM, comparable with vitamin C (EC50 = 15.77 μM).

 

Comments:

That's an intriguing study! Phenolic glycosides and lignan glycosides are compounds known for their potential health benefits, particularly their antioxidant and anti-inflammatory properties. It seems the researchers isolated several compounds from Gaultheria leucocarpa var. yunnanensis and evaluated their effects.

The compounds isolated (1-9) were likely subjected to various analytical techniques like NMR spectroscopy, mass spectrometry, and possibly X-ray crystallography to determine their structures. The experimental and calculated ECD (electronic circular dichroism) spectra are often used to confirm the configuration of chiral compounds.

The acid hydrolysis and enzymatic hydrolysis experiments might have been carried out to confirm the nature of the glycosidic linkages and to determine the aglycone part of the glycosides.

The anti-inflammatory and antioxidant activities of the compounds were evaluated using assays such as the inhibition of NO (nitric oxide) production induced by LPS (lipopolysaccharide) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, respectively.

Compounds 7 and 8 displayed promising inhibitory effects against LPS-induced NO production, with IC50 values of 63.71 μM and 10.66 μM, respectively. Comparing these values to L-NMMA (N^G-Monomethyl-L-arginine acetate), a known inhibitor, shows compound 8 to be particularly potent.

Compound 7 also exhibited significant DPPH radical scavenging activity with an EC50 of 18.75 μM, similar to the antioxidant activity of vitamin C (EC50 = 15.77 μM).

These findings suggest that compounds isolated from Gaultheria leucocarpa var. yunnanensis could potentially be used as natural sources for anti-inflammatory and antioxidant agents, which could have implications in the development of pharmaceuticals or nutraceuticals targeting such activities.

Related Products

Cat.No.	Product Name	Information
S7920	L-NMMA acetate	L-NMMA acetate (Tilarginine Acetate, Methylarginine acetate, NG-Monomethyl-L-arginine acetate, L-NG-monomethyl Arginine acetate) is an acetate salt form of L-NMMA. L-NMMA is a nitric oxide synthase(NOS) inhibitor with an IC50 of 4.1 μM for nNOS.

Related Targets

NOS