Mubritinib enhanced the inhibiting function of cisplatin in lung cancer by interfering with mitochondrial function

by Selleckchem | Mar 18 2023

Background: Lung cancer is one of the most lethal cancers worldwide. Cisplatin, a widely used anti-lung cancer drug, has been limited in clinical application due to its drug resistance. Medicines targeting mitochondrial electron transport chain (ETC) complexes may be effective candidates for cisplatin-based chemotherapy.

Methods: In this study, the small molecule drug library from Food and Drug Administration FDA was used to screen for medicines targeting ETC. MTT and colony formation assays were used to investigate cell proliferation. Flow cytometry was employed to analyze cell cycle, apoptosis, reactive oxygen species (ROS), and mitochondrial membrane potential. Wound scratch and transwell assays were used to detect migration and invasion abilities. The activities of the ETC complex were tested using kits. Western blot analysis was used to investigate the expressions of related proteins. A mouse xenograft model was constructed to verify the antitumor effect in vivo.

Results: The results showed that mubritinib can reduce the activation of the PI3K/mTOR signal pathway, disrupt mitochondrial function, significantly increase ROS levels and induce oxidative stress, and ultimately exert its antitumor effect against non-small cell lung cancer (NSCLC) both in vivo and in vitro. In addition, the combination of cisplatin and mubritinib can improve the tumor-suppressive effect of cisplatin.

Conclusion: Mubritinib can upregulate intracellular ROS concentration and cell apoptosis, inhibit the PI3K signaling pathway and interfere with the function of mitochondria, thus reducing cell proliferation and increasing ROS induced apoptosis by reducing the activation of Nrf2 by PI3K.

Comments：

This study aimed to identify medicines targeting mitochondrial electron transport chain complexes for cisplatin-based chemotherapy in lung cancer. The researchers screened the FDA small molecule drug library and found that mubritinib can reduce the activation of the PI3K/mTOR signal pathway, disrupt mitochondrial function, significantly increase ROS levels and induce oxidative stress, and ultimately exert its antitumor effect against non-small cell lung cancer (NSCLC) both in vivo and in vitro.

MTT and colony formation assays were used to investigate cell proliferation, and flow cytometry was employed to analyze cell cycle, apoptosis, ROS, and mitochondrial membrane potential. Wound scratch and transwell assays were used to detect migration and invasion abilities. The activities of the ETC complex were tested using kits. Western blot analysis was used to investigate the expressions of related proteins. A mouse xenograft model was constructed to verify the antitumor effect in vivo.

The combination of cisplatin and mubritinib can improve the tumor-suppressive effect of cisplatin. Mubritinib can upregulate intracellular ROS concentration and cell apoptosis, inhibit the PI3K signaling pathway and interfere with the function of mitochondria, thus reducing cell proliferation and increasing ROS-induced apoptosis by reducing the activation of Nrf2 by PI3K.

In conclusion, mubritinib may be a potential candidate for cisplatin-based chemotherapy in lung cancer, as it can target ETC complexes and interfere with mitochondrial function, ultimately leading to increased ROS levels and oxidative stress, which may contribute to its antitumor effect.