Home Blog of Signal Transduction Others Others Identification and application of a novel deoxynivalenol-degrading enzyme from Youhaiella tibetensis

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Identification and application of a novel deoxynivalenol-degrading enzyme from Youhaiella tibetensis

Deoxynivalenol (DON) poses a significant threat to human health due to its widespread distribution and biological toxicity. Here, we identified a novel DON-degrading enzyme from Youhaiella tibetensis (YoDDH). YoDDH exhibited the highest activity against DON at pH 4.5 and 40 ℃, in the presence of Ca2+ and the pyrroloquinoline quinone (PQQ). Additionally, YoDDH displayed remarkable thermostability at 40 ℃, with a half-life of 24 h and a Tm value of 48.5 ℃. Notably, phenazine methosulfate (PMS) and 2,6-dichlorophenolindophenol (DCPIP) can also serve as electron acceptors for YoDDH. After incubation in the optimal conditions for 3 h, YoDDH degraded 73 % of DON (100 μM) finally. The kcat and kcat /Km of YoDDH towards DON was determined as 1.65 s-1 and 1526 M-1·s-1 in the presence of PMS. The 3-keto-DON was verified as the degradation product. This identified YoDDH presents a promising candidate for DON decontamination in the food and feed industry.

 

Comments:

That's an intriguing discovery! YoDDH seems like a highly efficient enzyme capable of degrading DON, a mycotoxin commonly found in food and feed. Its optimal conditions at pH 4.5 and 40 ℃, along with its stability and ability to degrade DON by 73% within 3 hours, make it a promising tool for combating DON contamination.

The utilization of Ca2+ and PQQ to enhance YoDDH activity is interesting, as is its ability to utilize alternative electron acceptors like PMS and DCPIP. The determination of the degradation product, 3-keto-DON, is valuable for understanding the enzymatic mechanism involved in DON degradation.

If this enzyme can be effectively deployed in food and feed processing, it could significantly reduce the health risks associated with DON contamination. Its high kcat and kcat /Km values demonstrate its efficiency and potential for practical applications.

Further research and development could explore optimizing YoDDH for large-scale use in decontamination processes, ensuring its stability and effectiveness under various conditions encountered in the food and feed industry.

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