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Hypoxia-induced YAP activation and focal adhesion turnover to promote cell migration in mesenchymal TNBC cells

Background: Hypoxia is commonly characterized by malignant tumors that promote the aggressiveness and metastatic potential of cancer. Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, with approximately 46% capacity related to distant metastasis. Transcriptional factor yes-associated protein (YAP), a core component of the Hippo pathway, is associated with poor prognosis and outcome in cancer metastasis. Here, we explored the effect of hypoxia-mediated YAP activation and focal adhesions (FAs) turnover in mesenchymal TNBC cell migration.

Methods: We characterized the effect of hypoxia on YAP in different breast cancer cell lines using a hypoxia chamber and CoCl2 .

Results: Hypoxia-induced YAP nuclear translocation is significantly observed in normal breast epithelial cells, non-TNBC cells, mesenchymal TNBC cells, but not in basal-like TNBC cells. Functionally, we demonstrated that YAP activation was required for hypoxia to promote mesenchymal TNBC cell migration. Furthermore, hypoxia induced the localization of FAs at the leading edge of mesenchymal TNBC cells. In contrast, verteporfin (VP), a YAP inhibitor, significantly reduced the migration and the recruitment of nascent FAs at the cell periphery under hypoxia conditions, which only showed in mesenchymal TNBC cells.

Conclusions: Our data support the hypothesis that YAP is novel factor and positively responsible for hypoxia-promoting mesenchymal TNBC cell migration. Our findings provide further evidence and outcomes to help prevent the progression of TNBC.

Comments：Based on the study described, it appears that hypoxia-induced YAP activation is associated with mesenchymal TNBC cell migration. The study demonstrated that YAP activation was required for hypoxia to promote mesenchymal TNBC cell migration, and that verteporfin, a YAP inhibitor, reduced the migration and the recruitment of nascent FAs at the cell periphery under hypoxia conditions.

These findings suggest that YAP could be a novel factor responsible for promoting the aggressive behavior of mesenchymal TNBC cells under hypoxic conditions. The study provides important insights into the underlying mechanisms of TNBC metastasis, and may offer potential targets for preventing or treating the progression of TNBC.

Related Products

Cat.No. Product Name Information
S1786 Verteporfin Verteporfin is a small molecule that inhibits TEAD–YAP association and YAP-induced liver overgrowth. It is also a potent second-generation photosensitizing agent derived from porphyrin. Verteporfin is an autophagy inhibitor. Verteporfin inhibits cell proliferation and induces apoptosis.

Related Targets

TEAD Autophagy VDA YAP Apoptosis related