Hematological parameters, antioxidant status, and gene expression of γ-INF and IL-1β in vaccinated lambs fed different type of lipids

by Selleckchem | May 11 2023

This study was aimed to evaluate the effects of vegetable oils as calcium salt on immune responses and the expression of immune-related genes in vaccinated lambs. Twenty-four lambs (35 kg body weight, 6 months old) were assigned to four treatments with six replicates in a completely randomized design for 40 days. Four concentrates were formulated in which the calcium salts of palm oil, canola oil, corn oil, and flaxseed oil were used. On day 30 of the experiment, lambs were vaccinated by a dose of foot-and-mouth disease virus. The blood samples were collected from jugular vein 10 days after vaccination. The level of malondialdehyde and the activity of liver enzymes were the highest in lambs receiving corn oil and the lowest in lambs receiving flaxseed oil. The highest lymphocytes and the lowest neutrophil percentages were observed in lambs receiving flaxseed oil. There was a significant difference among treatments for the relative genes expression. Flaxseed oil significantly upregulated interferon-γ and corn oil upregulated interleukin-1β. The highest titer against foot-and-mouth disease virus was related to lambs receiving flaxseed oil, and the lowest titer was related to lambs that received corn oil. Flaxseed oil had more beneficial effects on immune response than other oils.

Comments：

The study aimed to investigate the effects of vegetable oils as calcium salt on the immune response and gene expression in vaccinated lambs. The study involved 24 six-month-old lambs, weighing 35 kg, assigned to four treatments with six replicates in a completely randomized design for 40 days.

Four concentrates were formulated with the calcium salts of palm oil, canola oil, corn oil, and flaxseed oil. On day 30, the lambs were vaccinated with foot-and-mouth disease virus, and blood samples were collected from the jugular vein 10 days after vaccination.

The results of the study showed that flaxseed oil had more beneficial effects on the immune response than the other oils. Lambs receiving corn oil had the highest levels of malondialdehyde and liver enzyme activity, while lambs receiving flaxseed oil had the lowest. Lambs receiving flaxseed oil also had the highest lymphocyte percentages and the lowest neutrophil percentages.

The relative gene expression showed a significant difference among treatments. Flaxseed oil significantly upregulated interferon-γ, while corn oil upregulated interleukin-1β. Lambs receiving flaxseed oil had the highest titer against foot-and-mouth disease virus, while those receiving corn oil had the lowest.

In summary, the study suggests that flaxseed oil may have more beneficial effects on the immune response than other vegetable oils. This information could be useful for farmers and animal feed manufacturers to optimize the use of vegetable oils in animal diets to improve their immune response.