Establishment of a Rapid and Effective Agrobacterium-Mediated Genetic Transformation System of Oxalis triangularis 'Purpurea'

Oxalis triangularis 'Purpurea' has significant ornamental value in landscaping. There is a critical necessity to elucidate the gene functions of O. triangularis 'Purpurea' and dissect the molecular mechanisms governing key ornamental traits. However, a reliable genetic transformation method remains elusive. In this study, our investigation revealed that various transformation parameters, including recipient material (petioles), pre-culture time (2-5 days), acetosyringone (AS) concentration (100-400 μM), Agrobacterium concentrations (OD600 = 0.4-1.0), infection time (5-20 min), and co-culture time (2-5 days), significantly impacted the stable genetic transformation in O. triangular 'Purpurea'. Notably, the highest genetic transformation rate was achieved from the leaf discs pre-cultured for 3 days, treated with 200 μM AS infected with Agrobacterium for 11 min at OD600 of 0.6, and subsequently co-cultured for 3 days. This treatment resulted in a genetic transformation efficiency of 9.88%, and it only took 79 days to produce transgenic plants. Our transformation protocol offers advantages of speed, efficiency, and simplicity, which will greatly facilitate genetic transformation for O. triangular 'Purpurea' and gene function studies.

 

Comments:

Your study seems incredibly thorough and detailed! Achieving a 9.88% genetic transformation rate for Oxalis triangularis 'Purpurea' with a refined protocol is a significant advancement. This efficiency and the relatively short time required for producing transgenic plants could indeed revolutionize genetic studies and trait modification in this plant species. Being able to identify the optimal parameters for genetic transformation—such as pre-culture time, concentrations of various agents, and duration of infection and co-culture—provides valuable insights for researchers in this field.

By establishing a reliable transformation method, your study sets the stage for dissecting gene functions and understanding molecular mechanisms that govern the ornamental traits of O. triangularis 'Purpurea'. It opens avenues for manipulating these traits more effectively, possibly leading to enhanced ornamental value or other beneficial characteristics in landscaping and horticulture.

This kind of breakthrough could have implications not only for O. triangularis 'Purpurea' but potentially for related plant species or other ornamental plants where genetic transformation has been challenging. This work has the potential to inspire similar studies aimed at improving transformation protocols for various plants, ultimately advancing our capabilities in genetic modification and trait enhancement across diverse species.

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