Electrospun nanofibers using β-cyclodextrin grafted chitosan macromolecules loaded with indomethacin as an innovative drug delivery system

by Selleckchem | Mar 06 2023

Electrospun nanofibers, as an innovative drug delivery system, provide selective, effective, and safe drug release. The present study aimed to fabricate nanofibers based on β-cyclodextrin grafted chitosan (β-CD-g-CS) macromolecules with incorporated drug via the blend electrospinning technique. The grafting of β-CD onto chitosan (CS) was confirmed by FT-IR, 1H NMR, TGA, XRD, and EDX analysis. Indomethacin was encapsulated in the β-CD-g-CS matrix as blend nanofibers using electrospinning in presence of polyvinyl alcohol (PVA). The SEM images revealed nanofibers with diameters at the nanoscale. The unique features of β-CD-g-CS/PVA as drug delivery system were investigated using indomethacin as a model drug molecule. Controlled release of indomethacin from nanofibers was studied in PBS solution by measuring the absorbance by UV-Vis spectrophotometer. The drug release profile exhibited that the rate of drug release can be tailored by polymer type and changing the drug/polymer ratio. The physicomechanical properties of the developed nanofibers were analyzed by tensile strength and water contact angle. The results demonstrated that β-CD-g-CS revealed enhanced wettability as well as favorable physicomechanical properties. In addition, the growth rate of the L929 cells on the CS and β-CD-g-CS nanofibers was not significantly inhibited and even improved cell proliferation. These findings indicated that β-CD-g-CS nanofibers could be appropriate as a smart drug delivery system for sustained release of indomethacin as an anti-inflammatory medicine in the wound healing and tissue engineering approaches in orthopedic applications.

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The study aimed to develop a new drug delivery system using electrospun nanofibers made from β-cyclodextrin grafted chitosan (β-CD-g-CS) macromolecules with incorporated indomethacin as a model drug molecule. The researchers confirmed the successful grafting of β-CD onto chitosan using various analytical techniques such as FT-IR, 1H NMR, TGA, XRD, and EDX analysis.

The resulting nanofibers had nanoscale diameters, as revealed by SEM images. The study also found that the release of indomethacin from the nanofibers could be controlled by altering the polymer type and changing the drug/polymer ratio. The release profile showed sustained drug release, indicating the potential of the nanofibers as a smart drug delivery system.

The physicomechanical properties of the nanofibers were evaluated by measuring tensile strength and water contact angle. The results showed that β-CD-g-CS had enhanced wettability and favorable physicomechanical properties, making it a promising candidate for use in wound healing and tissue engineering approaches in orthopedic applications.

Finally, the study assessed the biocompatibility of the nanofibers by evaluating the growth rate of L929 cells on both CS and β-CD-g-CS nanofibers. The results showed that cell proliferation was not significantly inhibited, and even improved on β-CD-g-CS nanofibers, indicating their biocompatibility.

In summary, the study demonstrated the potential of β-CD-g-CS nanofibers as a smart drug delivery system for sustained release of indomethacin and as a suitable material for orthopedic applications due to its favorable physicomechanical properties and biocompatibility.