Effects of staged multiple phytohormones application on capillary-driven attached Chlorella sp. biofilm

Comparing with single phytohormone application, applying multiple phytohormones to microalgae-based wastewater treatment systems can offer more extensive growth-promoting and stress-protecting effects for microalgae, yet the advantage of stress-relieving salicylic acid (SA) under combined phytohormones application scenario has not been exploited. Employing the improved capillary-driven attached microalgae culturing device (CD-PBR) previously used for single phytohormone application, this study compared the effects of mixed and single phytohormone(s) addition under as low as 10-7 M dosage. In order to make the best of SA for its stress-relieving property, postponed SA addition combined with applying other phytohormone(s) at the beginning of microalgae cultivation was also investigated. Combination of 10-6 M 6-benzylaminopurine (6-BA) with 10-7 M SA was sufficient for enhancing growth-promoting effects and anti-oxidative responses for attached Chlorella sp., while indole-3-acetic acid (IAA) addition was unnecessary. Combination of 6-BA addition at the beginning while postponed SA addition on Day 4 could further sustain such beneficial effects, while removing up to 99.7% total nitrogen (TN) and 97.9% total phosphorus (TP) from the bulk liquid. These results provided innovative strategies on mixed phytohormones addition for microalgae.

 

Comments:

This study delves into enhancing microalgae-based wastewater treatment by exploring the combined effects of different phytohormones at low concentrations. It seems the researchers aimed to optimize growth promotion and stress mitigation in microalgae, particularly Chlorella sp., using an improved culturing device.

The key findings suggest that a combination of 6-benzylaminopurine (6-BA) at a concentration of 10-6 M along with salicylic acid (SA) at 10-7 M was effective in stimulating growth and antioxidative responses in the attached Chlorella sp. They found that adding indole-3-acetic acid (IAA) wasn't necessary for these effects.

Moreover, an interesting discovery was made by postponing the addition of SA to Day 4 while starting with 6-BA at the beginning of cultivation. This strategy sustained the positive effects on growth and stress response while significantly reducing total nitrogen and phosphorus from the liquid.

This research showcases innovative strategies for optimizing microalgae growth in wastewater treatment systems, specifically by using combinations of phytohormones at minimal concentrations. The findings could potentially contribute to more efficient and environmentally friendly wastewater treatment methods.

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