Effect of Disulfiram on the Reproductive Capacity of Female Mice

In the process of assisted reproduction, the high-oxygen in vitro environment can easily cause oxidative damage to oocytes. Disulfiram (DSF) can play an anti-oxidant or pro-oxidant role in different cells, and the effect of DSF on oocytes remains unclear. Moreover, it remains unclear whether the use of DSF in the early stages of pregnancy has a negative impact on the fetus. In this study, we found that DSF increased serum FSH levels and increased the ovulation rate in mice. Moreover, DSF enhanced the antioxidant capacity of oocytes and contributed to the success rate of in vitro fertilization. Moreover, the use of DSF in early pregnancy in mice increased the uterine horn volume and the degree of vascularization, which contributed to a successful pregnancy. In addition, it was found that DSF regulated the mRNA expression of angiogenesis-related genes (VEGF), follicular development-related genes (C1QTNF3, mTOR and PI3K), ovulation-related genes (MAPK1, MAPK3 and p38 MAPK) and antioxidant-related genes (GPX4 and CAT). These results indicate that DSF is helpful for increasing the antioxidant capacity of oocytes and the ovulation rate. In early pregnancy in mice, DSF promotes pregnancy by increasing the degree and volume of uterine vascularization.

 

Comments：

This study provides evidence for the potential benefits of using disulfiram (DSF) in the process of assisted reproduction. The findings suggest that DSF can increase the ovulation rate and enhance the antioxidant capacity of oocytes, which can contribute to the success of in vitro fertilization. Additionally, the use of DSF in early pregnancy in mice increased the uterine horn volume and vascularization, which also contributes to successful pregnancy. The researchers also found that DSF regulates the expression of genes related to angiogenesis, follicular development, ovulation, and antioxidants, providing a potential mechanism for its observed effects.

It's important to note that this study was conducted in mice and further research is needed to determine if these results can be translated to humans. Additionally, more research is needed to fully understand the effects of DSF on fetal development and to determine the safety of using it during pregnancy.

 

Related Products

Cat.No.	Product Name	Information
S1680	Disulfiram	Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH) with IC50 of 0.15 μM and 1.45 μM for hALDH1 and hALDH2, respectively. Disulfiram is used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. Disulfiram induces apoptosis. Disulfiram is also an inhibitor of pore formation by gasdermin D (GSDMD).

Related Targets

Pyroptosis Dehydrogenase COVID-19 Apoptosis related