Development and validation of a liquid chromatography/tandem mass spectrometry assay for the simultaneous determination of dabigatran etexilate, intermediate metabolite and dabigatran in 50μL rat plasma and its application to pharmacokinetic study

by Selleckchem | Mar 17 2023

A simple, rapid and specific high performance liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for simultaneous determination of dabigatran etexilate (BIBR 1048 MS), the intermediate metabolite (BIBR 1087 SE) and dabigatran (BIBR 953 ZW). In this method, a stacked protein precipitation with methanol was performed in Sirocco 96-well filtration plates to extract analytes using only 50μL plasma. The analysis was performed on an Ultimate TM XB-C18 (4.6×50mm, 5μm) column using gradient elution with a mobile phase composed of methanol containing 0.01% formic acid and pure water at a flow rate of 0.3mL/min. The gradient was set to 90% methanol containing 0.01% formic acid for the first 1.0min, after which it dropped to 10%, and then was kept at 10% for the next 5min followed by an additional 1.0min at the initial composition of 90% methanol containing 0.01% formic acid for equilibration. Detection was performed on a triple-quadrupole mass spectrometer electrospray ionization interface in positive ion mode. Linear calibration curves were obtained over the concentration ranges of 1-500ng/mL for all analytes. The validated LC-MS/MS method for its selectivity, sensitivity, linearity, precision, accuracy, recovery, matrix effect and stability had been successfully applied to a pharmacokinetic study of analytes in rat plasma following a single oral administration of 15mg/kg dabigatran etexilate.

Comments：

A simple, rapid and specific high performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of dabigatran etexilate, an intermediate metabolite (BIBR 1087 SE) and dabigatran (BIBR 953 ZW) in rat plasma following a single oral administration of 15mg/kg dabigatran etexilate. The method involves a stacked protein precipitation with methanol in Sirocco 96-well filtration plates to extract analytes using only 50μL plasma. The analysis is performed on an Ultimate TM XB-C18 (4.6×50mm, 5μm) column using gradient elution with a mobile phase composed of methanol containing 0.01% formic acid and pure water at a flow rate of 0.3mL/min. The gradient is set to 90% methanol containing 0.01% formic acid for the first 1.0min, after which it drops to 10%, and then is kept at 10% for the next 5min followed by an additional 1.0min at the initial composition of 90% methanol containing 0.01% formic acid for equilibration. Detection is performed on a triple-quadrupole mass spectrometer electrospray ionization interface in positive ion mode.

Linear calibration curves are obtained over the concentration ranges of 1-500ng/mL for all analytes. The validated LC-MS/MS method is selective, sensitive, linear, precise, accurate, and stable, and has been successfully applied to a pharmacokinetic study of analytes in rat plasma following a single oral administration of 15mg/kg dabigatran etexilate. The method is therefore suitable for the determination of dabigatran etexilate and its metabolites in rat plasma for pharmacokinetic and other related studies.