Development and Characterization of Thiolated Cyclodextrin-Based Nanoparticles for Topical Delivery of Minoxidil

Purpose: The aim of this research was to prepare adhesive nanoparticles for the topical application of Minoxidil (MXD).

Methods: Thiolated β-CDs were prepared via conjugation of cysteamine with oxidized CDs. MXD was encapsulated within thiolated and unmodified β-CDs. Ionic gelation method was used to prepare nanoparticles (Thio-NP and blank NP) of CDs with chitosan. Nanoparticles were analyzed for size and zetapotential. Inclusion complexes were characterized via FTIR. Drug dissolution studies were carried out. An in vitro adhesion study over human hair was performed. An in vivo skin irritation study was performed. Ex vivo drug uptake was evaluated by using a Franz diffusion cell.

Results: Thiolated β-CDs presented 1804.68 ± 25 μmol/g thiol groups and 902.34 ± 25 μmol/g disulfide bonds. Nanoparticles displayed particle sizes within a range of 231 ± 07 nm to 354 ± 13 nm. The zeta potential was in the range of -8.1 ± 02 mV, +16.0 ± 05 mV. FTIR analyses confirmed no interaction between the excipients and drug. Delayed drug release was observed from Thio-NP. Thio-NP retained over hair surfaces for a significantly longer time. Similarly, drug retention was significantly improved. Thio-NP displayed no irritation over rabbit skin.

Conclusion: Owing to the above results, nanoparticles developed with MXD-loaded thiolated β-CDs might be a potential drug delivery system for topical scalp diseases.

 

Comments:

It seems like you're summarizing a scientific research paper or experiment related to the preparation of adhesive nanoparticles for topical application of Minoxidil. Here's a breakdown of the key points from your research:

**Objective:** The goal was to create adhesive nanoparticles for delivering Minoxidil topically.

**Methodology:**
- Thiolated β-CDs were produced by combining cysteamine with oxidized CDs.
- Minoxidil was encapsulated in both thiolated and unmodified β-CDs.
- Nanoparticles (Thio-NP and blank NP) of CDs with chitosan were prepared using the ionic gelation method.
- Analysis included assessing nanoparticle size, zeta potential, FTIR characterization of inclusion complexes, drug dissolution studies, in vitro adhesion studies on human hair, in vivo skin irritation studies on rabbits, and ex vivo drug uptake through Franz diffusion cells.

**Findings:**
- Thiolated β-CDs contained significant thiol groups and disulfide bonds.
- Nanoparticle sizes ranged from 231 nm to 354 nm, with zeta potential in the range of -8.1 mV to +16.0 mV.
- FTIR analysis indicated no interaction between the excipients and the drug.
- Delayed drug release was observed from Thio-NP.
- Thio-NP adhered longer to hair surfaces and enhanced drug retention.
- Thio-NP did not cause skin irritation in rabbit tests.

**Conclusion:**
- The study suggests that nanoparticles developed with Minoxidil-loaded thiolated β-CDs could serve as a potential drug delivery system for treating scalp diseases topically.

This research seems promising for developing an effective drug delivery system that enhances drug retention and minimizes irritation, especially for treating scalp-related conditions.

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