Caveolae-associated protein 3 promotes adipogenic differentiation of porcine preadipocytes by promoting extracellular signal-regulated kinase phosphorylation

by Selleckchem | Aug 10 2023

Fat deposition is one of the key factors affecting the economic development of pig husbandry. The aim of this study was to investigate the expression characteristics of caveolae-associated protein 3 (CAVIN3) and to elucidate its effect and mechanism on adipogenic differentiation of porcine preadipocytes. Cell transfection, quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blot, and oil red O staining were used to detect the effect of CAVIN3 on the differentiation of porcine preadipocytes. The results showed that CAVIN3 was expressed in various tissues, with higher expression in adipose tissue, differentially expressed during cell adipogenic differentiation, and mainly distributed in the cytoplasm. Functional studies showed that, after CAVIN3 interference in preadipocytes, the expression of adipogenic factors and the content of lipid droplets were significantly decreased (p < 0.05). The results were reversed after CAVIN3 was overexpressed. The mechanism research showed that LY3214996 inhibited the extracellular signal-regulated kinase (ERK) phosphorylation and further inhibited lipogenic factors expression. Overexpression of CAVIN3 attenuates the inhibitory effect of LY3214996 on ERK phosphorylation and attenuates its inhibitory effect on adipogenic differentiation. Therefore, this study demonstrated that CAVIN3 promotes the differentiation of porcine preadipocytes by promoting ERK phosphorylation. The present study can lay a theoretical foundation for further studying the molecular mechanism of porcine fat deposition.

Comments:

The study aimed to investigate the role of caveolae-associated protein 3 (CAVIN3) in adipogenic differentiation of porcine preadipocytes and its potential mechanism. Various experimental techniques were employed, including cell transfection, quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blotting, and oil red O staining.

The results of the study revealed that CAVIN3 was expressed in different tissues, with higher expression observed in adipose tissue. It was found to be differentially expressed during the adipogenic differentiation of cells and predominantly localized in the cytoplasm. Functional studies involving interference and overexpression of CAVIN3 in preadipocytes showed significant effects on adipogenic factors' expression and lipid droplet content. Specifically, when CAVIN3 was interfered with, the expression of adipogenic factors and lipid droplet content decreased significantly, while overexpression of CAVIN3 had the opposite effect.

Further investigation into the mechanism behind these effects revealed that LY3214996, a compound known to inhibit extracellular signal-regulated kinase (ERK) phosphorylation, also inhibited the expression of lipogenic factors. However, overexpression of CAVIN3 attenuated the inhibitory effect of LY3214996 on ERK phosphorylation and, consequently, its inhibitory effect on adipogenic differentiation.

Based on these findings, the study concluded that CAVIN3 promotes the differentiation of porcine preadipocytes by enhancing ERK phosphorylation. This research provides a theoretical basis for further exploration of the molecular mechanisms involved in porcine fat deposition, which could have implications for understanding and potentially manipulating fat deposition in pig husbandry, thereby impacting economic development in the field.