An efficient transformation method for tannin-containing sorghum

Background: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing.

Methods: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants.

Results: Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.

Comments：

It's also promising that the molecular analysis showed positive results for transgene expression, and the inheritance analysis confirmed that the transgenes could be inherited into the next generation.

Breaking the restriction imposed by tannins in plants could have significant implications for the agricultural industry. Sorghum is an important cereal crop used for food, feed, and bioenergy production, and its production can be limited by tannin accumulation, which reduces digestibility and palatability. By developing a method to efficiently transform tannin-containing sorghum, it could be possible to reduce or eliminate tannin accumulation, improving its nutritional value and increasing its potential as a food and feed crop.

It would be interesting to see how this transformation system could be further optimized to increase transformation frequency even more. Additionally, it would be valuable to see how this system could be applied to other tannin-containing crops to expand the possibilities of breaking the tannin restriction in plant transformation.

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S3028	Geneticin (G418 Sulfate)	Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.It is commonly used as a screening reagent for eukaryotic cells to screen transfected or transformed cells.

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