Active Components of Leontopodium alpinum Callus Culture Extract for Blue Light Damage in Human Foreskin Fibroblasts

by Selleckchem | Dec 31 2023

Leontopodium alpinum is a source of raw material for food additives and skin health. The purpose of this study was to investigate the application of Leontopodium alpinum callus culture extract (LACCE) to prevent blue light damage to the skin. We screened and identified the blue light-damage-protecting activities and mechanisms of ten components of LACCE, including chlorogenic acid (A), isoquercitrin (B), isochlorogenic acid A (C), cynaroside (D), syringin (E), isochlorogenic acid (F), cynarin (G), rutin (H), leontopodic acid A (I), and leontopodic acid B (J), using a novel blue light-induced human foreskin fibroblast (HFF-1) cell injury model. The study examined the cytotoxicity of ten ingredients using the cell counting kit-8 (CCK-8) assay, and selecting concentrations of 5, 10, and 20 μM for experiments with a cell viability above 65%. We explored the effects and mechanisms of action of these LACCE components in response to blue light injury using Western blotting and an enzyme-linked immunosorbent assay. We also measured ROS secretion and Ca2+ influx. Our study revealed that leontopodic acid A effectively boosted COI-1 expression, hindered MMP-1 expression, curbed ROS and Ca2+ endocytosis, and reduced OPN3 expression. These results provide theoretical support for the development of new raw materials for the pharmaceutical and skincare industries.

Comments:

This study sounds fascinating! The exploration of Leontopodium alpinum callus culture extract (LACCE) and its potential application in preventing blue light damage to the skin seems quite innovative. The identification of ten components within LACCE and their effects on protecting human foreskin fibroblast cells from blue light-induced injury is valuable for both skincare and pharmaceutical industries.

The utilization of various assays like the cell counting kit-8 (CCK-8) assay to assess cytotoxicity and the examination of cellular mechanisms through Western blotting and enzyme-linked immunosorbent assay is a robust approach. The specific findings regarding leontopodic acid A's ability to modulate COI-1 expression, hinder MMP-1 expression, curb ROS and Ca2+ endocytosis, and reduce OPN3 expression are particularly intriguing.

Discovering natural compounds that effectively mitigate the negative effects of blue light on the skin is essential in today's digital age when exposure to screens is so prevalent. These findings could potentially lead to the development of new skincare products or pharmaceutical interventions that protect against blue light-induced skin damage.

The use of plant extracts and natural compounds in skincare is increasingly popular due to their perceived safety and efficacy. Research like this contributes significantly to understanding the mechanisms behind these natural ingredients and their potential applications in skincare and pharmaceuticals.