A nanocellulose molecularly imprinted membrane: Preparation, characterization and application in targeted separation of taxane 10-deacetylbaccatin III

by Selleckchem | Nov 23 2023

Targeted separation of active phytochemicals is urgently needed in the natural medicine field. In this paper, due to the natural porosity and high biocompatibility of cellulose, a nanocellulose membrane combined with surface molecular imprinting was successfully prepared; the efficient nanocellulose-based molecular imprinted membrane (NC-MIM) provided good adsorption for the targeted separation of phytochemicals such as 10-deacetylbaccatin III (10-DAB), an essential intermediate in the synthesis of the anticancer drug paclitaxel. Through a series of characterization and adsorption experiments, the adsorption mechanism of NC-MIM was determined. At pH 8.0 and temperatures of 20 °C-40 °C, the maximum capacity of NC-MIM for adsorption of 10-DAB reached 66.90 mg g - 1, and the content of 10-DAB was dramatically increased 17.5-fold after adsorption. The specific adsorption results showed that NC-MIM had excellent capacity for targeted separation of 10-DAB from among taxane structural analogues. Even after ten cycles, NC-MIM demonstrated a remarkable adsorption capacity of 86.43 %, thereby indicating exceptional selectivity and stability. The successful implementation of NC-MIM for green, safe, and efficient enrichment of phytochemicals from plants provides a promising new approach and valuable insights into its practical application.

Comments:

The research described in the provided passage focuses on the development of a nanocellulose-based molecular imprinted membrane (NC-MIM) for the targeted separation of phytochemicals, specifically 10-deacetylbaccatin III (10-DAB), an important intermediate in the synthesis of the anticancer drug paclitaxel. This study is significant in the field of natural medicine, where the separation of active phytochemicals is crucial for various applications, including drug development.

Here's a breakdown of the key points in the passage:

### Research Focus:
The study addresses the need for targeted separation of active phytochemicals in natural medicine, with a specific focus on 10-DAB, an essential compound in paclitaxel synthesis.

### Methodology:
1. **Nanocellulose-Based Membrane:** A nanocellulose membrane was utilized due to its natural porosity and high biocompatibility.

2. **Surface Molecular Imprinting:** The nanocellulose membrane was combined with surface molecular imprinting, a technique where the template molecule (10-DAB) guides the formation of specific binding sites on the membrane.

### Results and Findings:
- **Adsorption Capacity:** The NC-MIM exhibited an impressive adsorption capacity for 10-DAB, reaching 66.90 mg g - 1 under specific conditions (pH 8.0, 20 °C-40 °C).

- **Selective Separation:** The membrane showed excellent specificity, separating 10-DAB from similar taxane structural analogues, indicating targeted separation ability.

- **Stability:** Even after ten adsorption-desorption cycles, the NC-MIM retained a remarkable adsorption capacity of 86.43%, indicating stability and reusability.

### Implications and Conclusion:
The successful development of NC-MIM demonstrates a green, safe, and efficient method for enriching phytochemicals from plants. This research not only provides a promising approach for targeted separation but also offers valuable insights into practical applications, particularly in the field of natural medicine and drug development.

In summary, this study presents a novel technique using nanocellulose-based molecular imprinting for the targeted separation of 10-DAB, showcasing its potential in the natural medicine field and potentially opening doors for further research and applications in pharmaceutical sciences.