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Uric Acid

Synonyms: 2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine

Uric Acid (2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine), a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.

Uric Acid Chemical Structure

Uric Acid Chemical Structure

CAS No. 69-93-2

Purity & Quality Control

Batch: S395501 0.5MNAOH]3.02 mg/mL]false]DMSO]0.01 mg/mL]false]Water]Insoluble]false Purity: 99.99%
99.99

Uric Acid Related Products

Biological Activity

Description Uric Acid (2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine), a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.
In vitro
In vitro Uric acid activates NFκB in a variety of cell culture models including proximal tubular cells. Uric acid suppresses 1-α hydroxylase mRNA and protein expression in dose dependent and time dependent manner[1].
Cell Research Cell lines HK2 cells
Concentrations 2.5, 5.0, 7.5, and 10 mg/dL
Incubation Time 1, 2, 4, 8, 16, and 24 hours
Method Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.
In Vivo
In vivo Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid. Uric acid is a strong reactive oxygen species (ROS) and peroxynitrite scavenger and antioxidant. Uric acid may exert fundamental roles in tissue healing via initiating the inflammatory process that is necessary for tissue repair, scavenging oxygen free radicals, and mobilizing progenitor endothelial cells[2].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05298891 Not yet recruiting
Acromegaly
Azienda Ospedaliero Universitaria Maggiore della Carita
 September 1 2024 Not Applicable
NCT06126315 Not yet recruiting
Amyotrophic Lateral Sclerosis
Mario Negri Institute for Pharmacological Research|University of Sydney|FightMND
 June 2024 Phase 2|Phase 3
NCT06177821 Recruiting
Prostatic Hyperplasia of the Medial Lobe
Seoul National University Hospital
 June 3 2024 Not Applicable
NCT06335537 Not yet recruiting
Uric Acid Stones
University of California Irvine
 May 2024 Phase 1

Chemical Information & Solubility

Molecular Weight 168.11 Formula

C5H4N4O3
CAS No. 69-93-2 SDF Download Uric Acid SDF
Smiles C12=C(NC(=O)N1)NC(=O)NC2=O
Storage (From the date of receipt)

In vitro
Batch:

0.5MNAOH : 3.02 mg/mL

DMSO : 0.01 mg/mL ( (0.05 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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