Uric Acid

Catalog No.S3955 Batch:S395501

Technical Data

Formula	C₅H₄N₄O₃
Molecular Weight	168.11	CAS No.	69-93-2
Solubility (25°C)*	In vitro	0.5MNAOH	3.02 mg/mL (17.96 mM)
		DMSO	0.01 mg/mL (0.05 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Uric Acid (2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine), a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.
In vitro	Uric acid activates NFκB in a variety of cell culture models including proximal tubular cells. Uric acid suppresses 1-α hydroxylase mRNA and protein expression in dose dependent and time dependent manner^[1].
In vivo	Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid. Uric acid is a strong reactive oxygen species (ROS) and peroxynitrite scavenger and antioxidant. Uric acid may exert fundamental roles in tissue healing via initiating the inflammatory process that is necessary for tissue repair, scavenging oxygen free radicals, and mobilizing progenitor endothelial cells^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HK2 cells Concentrations 2.5, 5.0, 7.5, and 10 mg/dL Incubation Time 1, 2, 4, 8, 16, and 24 hours Method Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.

Cell Assay:^{^[1]}

Cell lines
HK2 cells
Concentrations
2.5, 5.0, 7.5, and 10 mg/dL
Incubation Time
1, 2, 4, 8, 16, and 24 hours
Method
Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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