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L-Mimosine

Synonyms: Leucenol

L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.

L-Mimosine Chemical Structure

L-Mimosine Chemical Structure

CAS No. 500-44-7

Purity & Quality Control

L-Mimosine Related Products

Biological Activity

Description L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.
In vitro
In vitro L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. [1] L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. [2]
Cell Research Cell lines PC-3 and LNCaP cells
Concentrations ~800 μM
Incubation Time 48 hours
Method Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.
In Vivo
In vivo L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. [3]
Animal Research Animal Models Sprague-Dawley rats
Dosages ~50 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 198.18 Formula

C8H10N2O4
CAS No. 500-44-7 SDF Download L-Mimosine SDF
Smiles C1=CN(C=C(C1=O)O)CC(C(=O)O)N
Storage (From the date of receipt)

In vitro
Batch:

5%TFA : 3.04 mg/mL

DMSO : 0.01 mg/mL ( (0.05 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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