BAPTA-AM

Name: BAPTA-AM
Brand: Selleck Chemicals
SKU: S7534
Rating: 5 (74 reviews)

Catalog No.S7534 Purity:99.34%

BAPTA-AM is a selective, membrane-permeable calcium chelator.

BAPTA-AM Chemical Structure

CAS No. 126150-97-8

Purity & Quality Control

Batch: Purity: 99.34%

99.34

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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Express-Pick Library	Click to Expand

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SKOV3	Function assay		12 h	treatment with BAPTA-AM reduced the levels of [Ca2+]i	30819230
OVCAR3	Function assay		12 h	treatment with BAPTA-AM reduced the levels of [Ca2+]i	30819230
MC3T3-E1 cells	Function assay	2 μM	24 h	BAPTA/AM attenuated Tg(thapsigargin)-induced NADPH oxidase activity	27457075
HUVECs	Function assay	5 μmol/L	3 h	markedly suppressed Ca2+ increase	25643147
Click to View More Cell Line Experimental Data

Biological Activity

Description	BAPTA-AM is a selective, membrane-permeable calcium chelator.

In vitro
In vitro	BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. ^[1] BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. ^[2]
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	p-ERK / ERK		23185493

In Vivo
In vivo	BAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca²⁺ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats. ^[3]
Animal Research	Animal Models	BDL rats
	Dosages	~6 mg/kg
	Administration	i.p.

References

[4] Ni XC, et al. Redox Biol. 2022 Aug;54:102363.

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Chemical Information & Solubility

Molecular Weight	764.68	Formula	C₃₄H₄₀N₂O₁₈
CAS No.	126150-97-8	SDF	Download BAPTA-AM SDF
Smiles	CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 20 mg/mL ( (26.15 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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