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Hydroxytyrosol

Synonyms: 3-Hydroxytyrosol; 3,4-dihydroxyphenylethanol; Dihydroxyphenylethanol; 2-(3,4-Di-hydroxyphenyl)-ethanol

Hydroxytyrosol (3,4-dihydroxyphenylethanol) is one of the main phenolic components of olive oil with excellent antioxidant, antimicrobial and anticarcinogenic activities.

Hydroxytyrosol Chemical Structure

Hydroxytyrosol Chemical Structure

CAS No. 10597-60-1

Purity & Quality Control

Hydroxytyrosol Related Products

Biological Activity

Description Hydroxytyrosol (3,4-dihydroxyphenylethanol) is one of the main phenolic components of olive oil with excellent antioxidant, antimicrobial and anticarcinogenic activities.
In vitro
In vitro Hydroxytyrosol decreases the intracellular reactive oxygen species (ROS) level in MCF10A cells but not in MCF7 or MDA-MB-231 cells. Hydroxytyrosol prevents oxidative DNA damage in the three breast cell lines: MCF10A, MDA-MB-231 and MCF7 cells. It is a potent antioxidant because of its marked antioxidant activity, its ability to scavenge oxygen and nitrogen free radicals, to inhibit Low Density Lipoprotein (LDL) oxidation, platelet aggregation and endothelial cell activation and its protection against DNA damage. Hydroxytyrosol is able to reduce the synthesis of prostaglandin E2 blocking the transcription of COX-2 and 5-lipooxygenase, thereby reducing the chronic influence associated with diseases such as cancer[1].
Cell Research Cell lines MDA-MB-231, MCF10A and MCF7 cells
Concentrations 1 to 100 μM
Incubation Time 24 hours
Method Cells are seeded at 2 × 103 cells/well (MCF7) or 1 × 103 cells/well (MDA-MB-231 and MCF10A) into 96-well culture plates (flat bottom) (100 μL of cell suspension/well). At 24 h after plating, 100 μL of fresh culture medium, with different concentrations of HT is added in triplicate to the wells. Plates are incubated for 24 h or 24 h followed by a 48 h proliferation period with fresh medium at 37 °C and 5% CO2. At these time points, medium is removed and 200 μL of fresh RPMI medium without phenol red that contained XTT (200 μg/mL) and PMS (20 μg/mL) is added. Plates are incubated for 3 h at 37 °C in 5% CO2 and absorbance is measured at 450 nm wavelength (620 nm as reference) in a plate reader.
In Vivo
In vivo HT administration protects against hepatic I/R injury, as indicated by the decreased levels of serum aminotransferase and less parenchymal necrosis and apoptosis. Serum levels of tumor necrosis factor-α, interleukin-6, macrophage inflammatory protein 2, as well as reactive oxygen species (ROS) content in liver tissues, are all decreased by HT[2].
Animal Research Animal Models A mouse model of partial hepatic I/R injury (Male BALB/c mice)
Dosages 1 or 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 154.16 Formula

C8H10O3
CAS No. 10597-60-1 SDF Download Hydroxytyrosol SDF
Smiles C1=CC(=C(C=C1CCO)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 31 mg/mL ( (201.08 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 31 mg/mL

Ethanol : 31 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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