Ethidium bromide

Name: Ethidium bromide
Brand: Selleck Chemicals
SKU: S3689
Rating: 5 (6 reviews)

Synonyms: Homidium bromide, EtBr, EB

Catalog No.S3689 Purity:99.73%

Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Ethidium bromide Chemical Structure

CAS No. 1239-45-8

Purity & Quality Control

Batch: Purity: 99.73%

99.73

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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Express-Pick Library	Click to Expand

Biological Activity

Description	Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

In vitro
In vitro	Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr^[1].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06343311	Not yet recruiting	B-Cell Non-Hodgkin''s Lymphoma (NHL)\|Lymphoma Non-Hodgkins\|Lymphomas Non-Hodgkin''s B-Cell\|Non-Hodgkin Lymphoma\|Non-Hodgkin''s Lymphoma\|Large B-Cell Lymphoma\|Lymphoma Non-Hodgkin''s Adult\|Lymphoma\|Refractory Non-Hodgkin Lymphoma\|Relapsed Non-Hodgkin Lymphoma\|Lymphoma Non-Hodgkin\|HIV Associated Lymphoma\|CNS Lymphoma\|High-grade B-cell Lymphoma\|Refractory B-Cell Non-Hodgkin Lymphoma	Estrella Biopharma Inc.\|Eureka Therapeutics Inc.\|Estrella Immunopharma Inc.	May 15 2024	Phase 1\|Phase 2
NCT06073132	Recruiting	Generalized Epidermolysis Bullosa Simplex	TWi Biotechnology Inc.	April 2024	Phase 2\|Phase 3
NCT06378060	Recruiting	Severe Aplastic Anemia	Hematology department of the 920th hospital	March 1 2024	Phase 2
NCT05600140	Recruiting	Vision Disorders\|Vision Low	Royal Dutch Visio\|Radboud University Behavioural Science Institute Nijmegen The Netherlands	March 6 2024	Not Applicable
NCT06007235	Recruiting	Epidermolysis Bullosa Dystrophica\|Epidermolysis Bullosa Junctional	Organ Tissue Regeneration Repair and Replacement\|Euraxi Pharma\|ARANZ Medical	January 29 2024	Not Applicable
NCT05955326	Recruiting	Acute Cerebral Ischemia	Pharmazz Inc.	January 8 2024	Phase 4

References

[1] Sigmon J, et al. Electrophoresis. 1996, 17(10):1524-7.

Chemical Information & Solubility

Molecular Weight	394.31	Formula	C₂₁H₂₀N₃.Br
CAS No.	1239-45-8	SDF	Download Ethidium bromide SDF
Smiles	CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 78 mg/mL ( (197.81 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 7 mg/mL

Ethanol : 1 mg/mL

Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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