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Technical Data
| Formula | C21H20N3.Br |
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| Molecular Weight | 394.31 | CAS No. | 1239-45-8 | |
| Solubility (25°C)* | In vitro | DMSO | 78 mg/mL (197.81 mM) | |
| Water | 7 mg/mL (17.75 mM) | |||
| Ethanol | 1 mg/mL (2.53 mM) | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. |
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| In vitro | Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr[1]. |
Protocol (from reference)
Selleck's Ethidium bromide has been cited by 7 publications
| HIF-2α/TFR1 mediated iron homeostasis disruption aggravates cartilage endplate degeneration through ferroptotic damage and mtDNA release: A new mechanism of intervertebral disc degeneration [ J Orthop Translat, 2024, 46:65-78] | PubMed: 38808263 |
| The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation [ Signal Transduct Target Ther, 2023, 8(1):371] | PubMed: 37735446 |
| The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation [ Signal Transduct Target Ther, 2023, 8(1):371] | PubMed: 37735446 |
| Omarigliptin Protects the Integrity of the Blood-Brain Barrier After Intracerebral Hemorrhage in Mice [ J Inflamm Res, 2023, 16:2535-2548] | PubMed: 37342770 |
| cGAS-STING activation contributes to podocyte injury in diabetic kidney disease [ iScience, 2022, 25(10):105145] | PubMed: 36176590 |
| cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress [ FASEB J, 2022, 36(5):e22266] | PubMed: 35357035 |
| Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113] | PubMed: 30534421 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.