Pitstop 2

Name: Pitstop 2
Brand: Selleck Chemicals
SKU: S9670
Rating: 5 (7 reviews)

Catalog No.S9670 Purity:98.92%

Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin that inhibits both clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE).

Pitstop 2 Chemical Structure

CAS No. 1332879-52-3

Purity & Quality Control

Batch: S967001 DMSO]95 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.92%

98.92

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Biological Activity

Description

Targets

CIE ^[1]

In vitro
In vitro	Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin and inhibits clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE) in cells. The effects of pitstop 2 are not restricted to inhibition of clathrin since knockdown of clathrin fails to rescue the inhibition of endocytosis of CIE proteins by the drug. Thus pitstop 2 has additional cellular targets besides the amino terminal domain of clathrin and thus cannot be used to distinguish CIE from CDE.^[1]
Cell Research	Cell lines	Hela cells
	Concentrations	20 μM
	Incubation Time	15 min
	Method	Hela cells are preincubated with DMSO (control) or 20 μM pitstop 2 or pitstop 2-negative control for 15 min. Cells are allowed to internalize Alexa594-Transferrin and antibodies to MHCI for 30 min at 37℃ in the presence of DMSO or drugs. Surface bound antibodies are removed by low pH wash to visualize the internalized proteins (Internal) prior to fixation or cells are fixed immediately after the internalization to label for the internal and surface pools (Total) of the protein. Cells are then labeled with secondary antibodies to detect MHCI.

References

[1] Dipannita Dutta, et al. PLoS One. 2012;7(9):e45799.

Chemical Information & Solubility

Molecular Weight	473.36	Formula	C₂₀H₁₃BrN₂O₃S₂
CAS No.	1332879-52-3	SDF	--
Smiles	BrC1=CC=C(C=C1)\C=C2/SC(=NC2=O)N[S](=O)(=O)C3=CC=CC4=C3C=CC=C4
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 95 mg/mL ( (200.69 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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