Trabectedin

Name: Trabectedin
Brand: Selleck Chemicals
SKU: S7758
Rating: 5 (3 reviews)

Synonyms: Ecteinascidin 743, ET-743

Catalog No.S7758 Purity:99.92%

Trabectedin (Ecteinascidin 743, ET-743) is a marine alkaloid isolated from the Caribbean tunicate Ecteinascidia turbinata with antitumour activity. Trabectedin binds to the N2 of guanine in the minor groove, causing DNA damage and affecting transcription regulation in a promoter- and gene-specific manner.

Trabectedin Chemical Structure

CAS No. 114899-77-3

Purity & Quality Control

Batch: S775801 DMSO]100 mg/mL]false]]]false]]]false Purity: 99.92%

99.92

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Biological Activity

Description	Trabectedin (Ecteinascidin 743, ET-743) is a marine alkaloid isolated from the Caribbean tunicate Ecteinascidia turbinata with antitumour activity. Trabectedin binds to the N2 of guanine in the minor groove, causing DNA damage and affecting transcription regulation in a promoter- and gene-specific manner.

In vitro
In vitro	Trabectedin can block EWS-FLI1 transcription factor activity, suppress Werner syndrome protein (WRN) expression and selectively sensitize Ewing sarcoma cells to the DNA-damaging effects of SN38. <sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[3]</a></sup>
Cell Research	Cell lines	Ewing sarcoma cells
	Concentrations	10 nM
	Incubation Time	18 h
	Method	RNAiMax Lipofectamine (4.5 mL per well in a 6-well dish) was added to siRNA targeting the EWS-FLI1 breakpoint site II, complexed, combined with cells, and incubated for 36 to 48 hours before collection for Western blot analysis.

In Vivo
In vivo	In Tumor-Bearing Mice, trabectedin does not have an impact on the functional activity of MDSC but does significantly reduce the number of Ly6C<sup>high</sup> cells, the most suppressive subset. <sup><a class="sref" href="#s_ref">[4]</a></sup>

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06050434	Recruiting	Soft Tissue Sarcoma	Institut für Klinische Krebsforschung IKF GmbH at Krankenhaus Nordwest\|PharmaMar	October 31 2023	--
NCT05868889	Completed	Ovarian Cancer	Grupo Español de Investigación en Cáncer de Ovario	October 21 2021	--
NCT05597917	Recruiting	Soft Tissue Sarcoma	Universität Münster\|Anturec Pharmaceuticals GmbH	October 26 2021	Phase 3
NCT04979442	Terminated	Dedifferentiated Liposarcoma	Rain Oncology Inc	July 14 2021	Phase 3

References

[4] Germano G, et al. Cancer Cell. 2013 Feb 11;23(2):249-62.

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Chemical Information & Solubility

Molecular Weight	761.84	Formula	C₃₉H₄₃N₃O₁₁S
CAS No.	114899-77-3	SDF	--
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
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DMSO : 100 mg/mL ( (131.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

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