Home Metabolism Other inhibitor HQNO For research use only.

HQNO

Synonyms: 2-heptyl-4-hydroxyquinoline-N-oxide

HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.

HQNO Chemical Structure

HQNO Chemical Structure

CAS No. 341-88-8

Purity & Quality Control

Batch: S897001 Ethanol]16 mg/mL]false]DMSO]9 mg/mL]false]Water]Insoluble]false Purity: 99.31%
99.31

HQNO Related Products

Biological Activity

Description HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.
Targets
Complex III [1]
(Cell-free assay)		 NDH-2 [2]
(Cell-free assay)
64 nM(Kd) 7.3 μM
In vitro
In vitro

The aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor (designated NDH-2). The respiratory inhibitor HQNO inhibits the activity of NADH:ubiquinone-1 oxidoreductase and NADH:menadione oxidoreductase by about 40% and 45%, respectively, at a concentration of 20 μM.[3]
Cell Research Cell lines Bacillus cereus KCTC 3674
Concentrations 10 μM, 20 μM, 30 μM, 40 μM, 50 μM
Incubation Time --
Method

The assay mixture of NADH:ubiquinone-1 oxidoreductase containes a Triton X-100 extract of B. cereus membranes (10 μg protein), 30 mM KCN, and 50 μM Q-1 in 2 ml of 50 mM MES-KOH (pH 6.0) containing 0.1M NaCl. The activity of NADH: ubiquinone-1 oxidoreductase is measured with various concentrations of HQNO.

Chemical Information & Solubility

Molecular Weight 259.34 Formula

C16H21NO2
CAS No. 341-88-8 SDF --
Smiles CCCCCCCC1=CC(=O)C2=CC=CC=C2N1O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Ethanol : 16 mg/mL

DMSO : 9 mg/mL ( (34.7 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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