Coomassie Blue R-250

Name: Coomassie Blue R-250
Brand: Selleck Chemicals
SKU: E2704
Rating: 5 (1 reviews)

Synonyms: Brilliant Blue R250

Catalog No.E2704 Purity:99%

Coomassie Blue R-250 (Brilliant blue R250), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

Coomassie Blue R-250 Chemical Structure

CAS No. 6104-59-2

Purity & Quality Control

Batch: E270401 DMSO]100 mg/mL]false]Ethanol]3 mg/mL]false]Water]2 mg/mL]false Purity: 99%

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Biological Activity

Description	Coomassie Blue R-250 (Brilliant blue R250), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

References

[1] Krause RGE, et al. Anal Biochem. 2019 Feb 1;566:107-115.

Chemical Information & Solubility

Molecular Weight	827.98	Formula	C₄₅H₄₅N₃O₇S₂.Na
CAS No.	6104-59-2	SDF	--
Smiles	[Na;v0].CCOC1=CC=C(NC2=CC=C(C=C2)C(C3=CC=C(C=C3)N(CC)CC4=CC(=CC=C4)[S;v6](O)(=O)=O)=C5C=CC(C=C5)=[N+;v4](CC)CC6=CC(=CC=C6)[S;v6]([O-])(=O)=O)C=C1
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (120.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : 2 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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