Home Others Other chemical Brassinolide For research use only.

Brassinolide

Brassinolide, a plant steroid isolated from Brassica napus pollen, promotes cell elongation and cell devision and thus regulates plant growth and development.

Brassinolide Chemical Structure

Brassinolide Chemical Structure

CAS No. 72962-43-7

Purity & Quality Control

Brassinolide Related Products

Biological Activity

Description Brassinolide, a plant steroid isolated from Brassica napus pollen, promotes cell elongation and cell devision and thus regulates plant growth and development.
In vitro
In vitro Brassinolide is a naturally occurring plant hormone that promotes growth, increases yields for grain and fruit crops, and makes plants more resistant to drought and cold weather. Brassinolide could induce a time and concentration-dependent cytotoxicity in PC-3 cells. Treatment with brassinolide triggers a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2 and marked accumulation in G2/M phase of cell cycle[1].
Cell Research Cell lines PC-3 cells
Concentrations 0, 10, 20 and 40 μM
Incubation Time 12, 24 and 36 h
Method

PC-3 cells are treated with various concentrations of brassinolide (0, 10, 20 and 40 μM) for 12, 24 and 36 h.

Chemical Information & Solubility

Molecular Weight 480.68 Formula

C28H48O6
CAS No. 72962-43-7 SDF --
Smiles CC(C)C(C)C(C(C(C)C1CCC2C1(CCC3C2COC(=O)C4C3(CC(C(C4)O)O)C)C)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 96 mg/mL ( (199.71 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 24 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Brassinolide | Brassinolide supplier | purchase Brassinolide | Brassinolide cost | Brassinolide manufacturer | order Brassinolide | Brassinolide distributor