5-Hydroxymethylcytosine

Name: 5-Hydroxymethylcytosine
Brand: Selleck Chemicals
SKU: E0027
Rating: 5 (1 reviews)

Synonyms: 5hmC

Catalog No.E0027 Purity:99.89%

5-Hydroxymethylcytosine (5hmC) is a naturally existing component in mammalian genomic DNA and is regarded as the sixth DNA base. 5-Hydroxymethylcytosine is an intermediate metabolite in active DNA demethylation. 5-Hydroxymethylcytosine is epigenetic marks known to affect global gene expression in mammals.

5-Hydroxymethylcytosine Chemical Structure

CAS No. 1123-95-1

Purity & Quality Control

Batch: E002701 DMSO]200 mg/mL]true]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.89%

99.89

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Related Compound Libraries	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Highly Selective Inhibitor Library	Click to Expand

Biological Activity

Description	5-Hydroxymethylcytosine (5hmC) is a naturally existing component in mammalian genomic DNA and is regarded as the sixth DNA base. 5-Hydroxymethylcytosine is an intermediate metabolite in active DNA demethylation. 5-Hydroxymethylcytosine is epigenetic marks known to affect global gene expression in mammals.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT04741945	Recruiting	Preleukemia\|Myelodysplastic Neoplasm\|Cytopenia\|Preleukemic Anemia	Kirsten Grønbæk\|Steno Diabetes Center Copenhagen\|Zealand University Hospital\|Van Andel Research Institute\|Herlev Hospital\|Technical University of Denmark\|Region Hovedstadens Apotek\|University of Copenhagen\|Rigshospitalet Denmark	December 13 2021	Phase 2
NCT05318989	Completed	Genitourinary Agents	Żelazna Medical Centre LLC\|Laboratory of Genetics and Human Genomics University of Gdańsk	February 8 2021	--
NCT03919539	Unknown status	Drug Resistance to Famitinib and Camrelizumab\|Biomarkers for Efficacy and Toxicity	Peking University People''s Hospital\|Peking University\|Northwestern University	December 1 2019	--
NCT03999723	Recruiting	Myelodysplastic Syndromes\|Acute Myeloid Leukemia\|Chronic Myelomonocytic Leukemia	Kirsten Grønbæk\|Van Andel Institute - Stand Up To Cancer Epigenetics Dream Team\|Karolinska University Hospital\|Skane University Hospital\|Sahlgrenska University Hospital Sweden\|University of Southern California\|Imperial College London\|University of Copenhagen\|Zealand University Hospital\|Aalborg University Hospital\|Odense University Hospital\|Technical University of Denmark\|Aarhus University Hospital\|Uppsala University Hospital\|Rigshospitalet Denmark	September 11 2019	Phase 2
NCT03526666	Completed	Myelodysplastic Syndromes\|Acute Myeloid Leukemia\|Chronic Myelomonocytic Leukemia\|MDS\|AML\|CMML	Van Andel Research Institute	November 1 2017	--

References

Chemical Information & Solubility

Molecular Weight	141.13	Formula	C₅H₇N₃O₂
CAS No.	1123-95-1	SDF	--
Smiles	NC1=C(CO)C=NC(=O)N1
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 200 mg/mL ( (1417.13 mM) Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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Molarity Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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