3-Amino-9-ethylcarbazole

Name: 3-Amino-9-ethylcarbazole
Brand: Selleck Chemicals
SKU: E2703
Rating: 5 (1 reviews)

Synonyms: AEC

Catalog No.E2703 Purity:98.70%

3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained with HRP-conjugated secondary antibodies.

3-Amino-9-ethylcarbazole Chemical Structure

CAS No. 132-32-1

Purity & Quality Control

Batch: E270301 DMSO]42 mg/mL]false]Ethanol]21 mg/mL]false]Water]Insoluble]false Purity: 98.70%

98.70

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Biological Activity

Description	3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained with HRP-conjugated secondary antibodies.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06388889	Enrolling by invitation	Eosinophilic Asthma	Areteia Therapeutics	May 8 2024	Phase 3
NCT05813288	Recruiting	Eosinophilic Asthma\|Asthma; Eosinophilic\|Asthma	Areteia Therapeutics	March 27 2023	Phase 3
NCT05748600	Recruiting	Eosinophilic Asthma\|Asthma; Eosinophilic\|Asthma Attack	Areteia Therapeutics	January 30 2023	Phase 3
NCT05763121	Recruiting	Eosinophilic Asthma\|Asthma; Eosinophilic\|Asthma	Areteia Therapeutics	January 30 2023	Phase 3
NCT04128371	Terminated	Eosinophilia\|Angioedema	National Institute of Allergy and Infectious Diseases (NIAID)\|National Institutes of Health Clinical Center (CC)	January 14 2021	Phase 2
NCT04191304	Recruiting	Hypereosinophilic Syndrome	AstraZeneca	July 20 2020	Phase 3

References

[1] Stack EC, et al. Methods. 2014 Nov;70(1):46-58.

Chemical Information & Solubility

Molecular Weight	210.27	Formula	C₁₄H₁₄N₂
CAS No.	132-32-1	SDF	--
Smiles	CC[N]1C2=CC=CC=C2C3=CC(=CC=C13)N
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 42 mg/mL ( (199.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 21 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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