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Madecassoside

Synonyms: Asiaticoside A

Madecassoside (Asiaticoside A) is the main active triterpene constituent of Centella asiatica herbs, a traditional Chinese medicine for wound healing and scar management.

Madecassoside Chemical Structure

Madecassoside Chemical Structure

CAS No. 34540-22-2

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Madecassoside Related Products

Biological Activity

Description Madecassoside (Asiaticoside A) is the main active triterpene constituent of Centella asiatica herbs, a traditional Chinese medicine for wound healing and scar management.
In vitro
In vitro Madecassoside (10, 30, 100 μmol/L) could reverse morphological changes, elevate cell viability, increase glutathione levels, and decrease lactate dehydrogenase andmalondialdehyde levels caused by H2O2 in a concentration-dependent manner. It attenuates apoptosis, preventing the activation of caspase-3 and the loss of mitochondria membrane potential, as well as the phosphorylation of p38 mitogen activated protein kinase (MAPK) in HUVECs. Hence, Madecassoside could protect HUVECs against lipid peroxidation and apoptosis caused by H2O2[1].
Cell Research Cell lines human umbilical vein endothelial cells (HUVECs)
Concentrations 10, 30, 100 μmol/L
Incubation Time 8 h
Method

HUVECs are harvested using 0.25% trypsin solution and inoculated in 96-well plates at a density of 1 × 104 cells/well. Cells are treated with H2O2 (500 μmol/L) and/or a variety of concentrations of madecassoside (10, 30, 100 μmol/L). The culture plate is then placed in 5% (v/v) CO2 at 37◦C for 8 h. At the end of incubation, 20 μL of MTT (5 mg/mL) is added to each well and the cells are incubated for another 4 h at 37◦C with 5% CO2. The supernatants are then discarded, and 200 μL of DMSO is added to each well. The absorbance is read at 540 nm and cell viability is expressed as percentage of untreated cells.
In Vivo
In vivo The pharmacokinetic study of madecassoside in rats indicates that the maximum plasma concentration is 303.75 ± 28.53 ng/mL after single oral administration (100 mg/kg). Madecassoside, administered orally for 20 days at doses of 6, 12, and 24 mg/kg, effectively facilitates burn wound healing in mice. It could reduce the burn wound area, leading to a better healing pattern with almost complete wound closure in mice[1]. Madecassoside has potent anti-pulmonary fibrosis (PF) effects when administered p.o., despite having extremely low oral bioavailability. The potent anti-PF effects induced by p.o. madecassoside in mice are not mediated by its metabolites or itself after absorption into blood. i.p. madecassoside has no anti-PF effect. Madecassoside increases the activity of PPAR-γ, which subsequently increases HGF expression in colonic epithelial cells. HGF then enters into the circulation and lung tissue to exert an anti-PF effect[2].
Animal Research Animal Models Female ICR mice, 6-8 weeks-old and weighing 22-26 g
Dosages 40 mg/kg
Administration p.o. or i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02353273 Completed
Diabetic Foot
Oneness Biotech Co. Ltd.
 July 22 2015 Phase 1

Chemical Information & Solubility

Molecular Weight 975.12 Formula

C48H78O20
CAS No. 34540-22-2 SDF --
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (102.55 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL


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In vivo
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