Home Immunology & Inflammation Immunology & Inflammation related chemical - NF-κB modulator - HIF modulator - ROS modulator N-Acetylcysteine amide For research use only.

N-Acetylcysteine amide

N-acetylcysteine amide is a membrane penetrating antioxidant with anti-inflamatory activity through regulation of activation of NF-κB and HIF-1α as well as modulation of ROS.

N-Acetylcysteine amide Chemical Structure

N-Acetylcysteine amide Chemical Structure

CAS No. 38520-57-9

Purity & Quality Control

N-Acetylcysteine amide Related Products

Biological Activity

Description N-acetylcysteine amide is a membrane penetrating antioxidant with anti-inflamatory activity through regulation of activation of NF-κB and HIF-1α as well as modulation of ROS.
Targets
NF-κB [1] HIF-1α [1] ROS [1]
In vitro
In vitro

The antioxidant N-acetylcysteine (NAC) blocks erastin-induced or RSL3-induced cell death in Nupr1-/- mPDAC cells, an effect that is associated with decreased production or release of MDA, 8-OHdG, or HMGB1. NUPR1-knockdown PANC1 cells exhibits increased MDA, 8-OHdG, and HMGB1 release during ferroptotic cell death, which can be reversed by the addition of NAC.[2]
Cell Research Cell lines mPDAC cells
Concentrations 1 mM
Incubation Time 24 h
Method

Indicated mPDAC cells are treated with erastin (10 μM) or RSL3 (1 μM) in the absence or presence of DFO (100 M) or N-acetylcysteine (1 mM) for 24 h, and then intracellular MDA, intracellular 8-OHdG, extracellular HMGB1, and cell viability are assayed by a CCK8 kit.
In Vivo
In vivo

Combined n-acetylcysteine (NAC) and bone marrow-derived mesenchymal stem cells (BMSCs) therapy alleviates oxidative stress damage to the pancreas and inhibits the inflammatory response to a significantly greater extent than single therapy with either BMSCs or NAC.[3]

Chemical Information & Solubility

Molecular Weight 162.21 Formula

C5H10N2O2S
CAS No. 38520-57-9 SDF --
Smiles CC(=O)NC(CS)C(=O)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 32 mg/mL ( (197.27 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 32 mg/mL

Ethanol : 16 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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