N-Acetylcysteine amide

Catalog No.S5804 Batch:S580405

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Technical Data

Formula

C5H10N2O2S
Molecular Weight 162.21 CAS No. 38520-57-9
Solubility (25°C)* In vitro DMSO 32 mg/mL (197.27 mM)
Water 32 mg/mL (197.27 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description N-acetylcysteine amide is a membrane penetrating antioxidant with anti-inflamatory activity through regulation of activation of NF-κB and HIF-1α as well as modulation of ROS.
Targets
NF-κB [1] HIF-1α [1] ROS [1]
In vitro

The antioxidant N-acetylcysteine (NAC) blocks erastin-induced or RSL3-induced cell death in Nupr1-/- mPDAC cells, an effect that is associated with decreased production or release of MDA, 8-OHdG, or HMGB1. NUPR1-knockdown PANC1 cells exhibits increased MDA, 8-OHdG, and HMGB1 release during ferroptotic cell death, which can be reversed by the addition of NAC.[2]
In vivo

Combined n-acetylcysteine (NAC) and bone marrow-derived mesenchymal stem cells (BMSCs) therapy alleviates oxidative stress damage to the pancreas and inhibits the inflammatory response to a significantly greater extent than single therapy with either BMSCs or NAC.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    mPDAC cells

  • Concentrations

    1 mM

  • Incubation Time

    24 h

  • Method

    Indicated mPDAC cells are treated with erastin (10 μM) or RSL3 (1 μM) in the absence or presence of DFO (100 M) or N-acetylcysteine (1 mM) for 24 h, and then intracellular MDA, intracellular 8-OHdG, extracellular HMGB1, and cell viability are assayed by a CCK8 kit.

Selleck's N-Acetylcysteine amide has been cited by 14 publications

Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
Base-excision repair pathway regulates transcription-replication conflicts in pancreatic ductal adenocarcinoma [ Cell Rep, 2024, 43(10):114820] PubMed: 39368091
A disulfidptosis-related lncRNA signature for analyzing tumor microenvironment and clinical prognosis in hepatocellular carcinoma [ Front Immunol, 2024, 15:1412277] PubMed: 39434887
ABT‑737 increases cisplatin sensitivity through the ROS‑ASK1‑JNK MAPK signaling axis in human ovarian cancer cisplatin‑resistant A2780/DDP cells [ Oncol Rep, 2024, 52(3)122] PubMed: 39054955
Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons [ Nat Commun, 2023, 14(1):5165] PubMed: 37620297
Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons [ Nat Commun, 2023, 14(1):5165] PubMed: 37620297
Omicron Spike Protein is Vulnerable to Reduction [ J Mol Biol, 2023, 435(13):168128] PubMed: 37100168
Omicron Spike Protein is Vulnerable to Reduction [ J Mol Biol, 2023, 435(13):168128] PubMed: 37100168
Comprehensive analysis of cuproptosis-related lncRNAs for prognostic significance and immune microenvironment characterization in hepatocellular carcinoma [ Front Immunol, 2022, 13:991604] PubMed: 36685508
NUPR1 is a critical repressor of ferroptosis [ Nat Commun, 2021, 12(1):647] PubMed: 33510144

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.