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Demethylzeylasteral (T-96) Immunology & Inflammation related inhibitor

Name: Demethylzeylasteral (T-96)
Brand: Selleck Chemicals
SKU: S3608
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S3608

An active component isolated from Tripterygium wilfordii Hook F., Demethylzeylasteral (T-96) inhibits UDP-glucuronosyltransferase (UGT) isoforms UGT1A6 and UGT2B7 with immunosuppressive effects.

Chemical Structure

Molecular Weight: 480.59

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Quality Control

Batch: Purity: 99.97%

99.97

Related Targets	PD-1/PD-L1 CXCR STING AhR CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor
Other Immunology & Inflammation related Products	Cl-amidine Tempol Anti-mouse CD8α-InVivo Tranilast Sinomenine Geniposidic acid GI254023X (GI4023) CORM-3 Bestatin (Ubenimex) Acacetin

Solubility

In vitro
Batch:

DMSO : 96 mg/mL (199.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 33 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (10.40mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	480.59	Formula	C₂₉H₃₆O₆	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	107316-88-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC12CCC(CC1C3(CCC4(C5=CC(=C(C(=C5C(=O)C=C4C3(CC2)C)C=O)O)O)C)C)(C)C(=O)O

Mechanism of Action

Targets/IC₅₀/Ki	UGT1A6 (Cell-free assay) 0.6 μM(Ki) UGT2B7 (Cell-free assay) 17.3 μM(Ki)
In vitro	Demethylzeylasteral (T-96) shows strong inhibition towards UGT1A6 and UGT2B7, with negligible influence towards UGT1A9. It has an anti-tumor property in melanoma cells, not only inhibiting cell proliferation through cell cycle arrest at S phase, but also inducing cell apoptosis. CDK2 and Cycin E1 decrease in a dose-dependent manner after its treatment. This compound inhibits MCL1, whose overexpression recovers the proliferation ability inhibited by demethylzeylasteral. It also inhibits clonogenicity and tumorigenesis in melanoma cells through downregulating the expression of MCL1. Furthermore, it is found to inhibit Ca2+ currents in mouse spermatogonia and has an antifertility ability.
In vivo	Demethylzeylasteral (T-96) demonstrates a significant proteinuria reduction both in a time and concentration-dependent manner. It significantly inhibits the activation of NF-kB in the kidneys of MRL/lpr mice. In addition, this compound reduces the secretion of pro-inflammatory mediators such as TNF-α, COX-2 and ICAM-1.
References	[1] https://pubmed.ncbi.nlm.nih.gov/22874791/ [2] https://pubmed.ncbi.nlm.nih.gov/29072681/ [3] https://pubmed.ncbi.nlm.nih.gov/26208003/

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