Home Immunology & Inflammation Immunology & Inflammation related inhibitor Atractylenolide I For research use only.

Atractylenolide I

Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.

Atractylenolide I Chemical Structure

Atractylenolide I Chemical Structure

CAS No. 73069-13-3

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Atractylenolide I Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells Function assay Transactivation of p53 in human HepG2 cells expressing MDM2 promoter by transient transfection reporter assay 22365756
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Biological Activity

Description Atractylenolide I is the major sesquiterpenoid of the rhizome of A. macrocephala and shows a wide spectrum of pharmacological activities such as antiinflammatory, digestion promoting, and antioxidant effects.
In vitro
In vitro Atractylenolide I decreases the TNF-α level in LPS-stimulated peritoneal macrophages in a dosedependent manner, IC50=23.1 μM. It also inhibits NO production in LPS-activated peritoneal macrophages, IC50=41 μM. The IC50 of Atractylenolide I for inhibiting the activity of iNOS is 67.3 μM. It shows no marked effects against 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). Atractylenolide I is also reported previously to act on white blood cell membranes and TLR4 and its antiinflammatory activity is related to antagonizing TLR4 [1].
Cell Research Cell lines peritoneal macrophages
Concentrations 1-1000 μM
Incubation Time 24 h
Method 2 × 104 cells per well are plated into 96-well plates, cells/well to a final volume of 200 μL, incubated at 37 °C for 24 h, and given a fresh change of medium. The cells are treated with atractylenolide I (1-1000 μM) dissolved in DMSO, and with LPS (final concentration 10 μg/mL) alone or in combination with atractylenolide I (1–100 μM) dissolved in DMSO. This DMSO percentage allows the optimal solubilization of atractylenolide I in aqueous solution. The control and LPS wells receive the same amount of DMSO. After 24 h of incubation at 37 °C, 20 μL of MTT (5 mg/mL) is added to each well and 4 h later the cells are lysed with 150 μL DMSO. The plate is shaken for 10 min and 30 min later the optical densities (OD570) for atractylenolide I are compared with the OD of the control or LPS stimulated wells to assess the cytotoxicity.
In Vivo
In vivo Atractylenolide I has anti-inflammatory effects. The treatment of Atractylenolide I significantly attenuates LPS-induced lung wet-to-dry weight ratio and MPO activity in LPS-induced acute lung injury mouse. It also significantly inhibits the production of TNF-α, IL-6, IL-1β, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF, upregulates the production of IL-10 in BALF. Atractylenolide I protects mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation[2].
Animal Research Animal Models LPS-induced acute lung injury mouse
Dosages 5, 10 and 20 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 230.30 Formula

C15H18O2
CAS No. 73069-13-3 SDF Download Atractylenolide I SDF
Smiles CC1=C2CC3C(=C)CCCC3(C=C2OC1=O)C
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