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Thymoquinone

Name: Thymoquinone
Brand: Selleck Chemicals
SKU: S4761
Rating: 5 (3 reviews)

Catalog No.S4761 Purity:99.74%

Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.

Thymoquinone Chemical Structure

CAS No. 490-91-5

Purity & Quality Control

Batch: Purity: 99.74%

99.74

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Biological Activity

Description	Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.

In vitro
In vitro	Thymoquinone (TQ) strongly inhibits the production of nitric oxide (NO) and repressed NO synthase (iNOS), tumor necrosis factor (TNF)-α, cyclooxygenase (COX)−2, interleukin (IL)−6, and IL-1β expression in LPS-activated RAW264.7 cells. TQ acts as a radical scavenger and thereby preserves the enzymatic activity of antioxidants like catalase, glutathione peroxidase, and glutathione-S-transferase. TQ abolishes the activity of IRAK1, but not IRAK4, thereby reducing the activity of downstream NF-κB and AP-1^[1]. Treatment with TQ significantly decreases cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment significantly decreases the level of phosphorylated p65 in the nucleus, which indicates the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreases the expression levels of VEGF, c-Myc and Bcl-2^[2].
Cell Research	Cell lines	RAW264.7 cells, peritoneal macrophages, and HEK293 cells
	Concentrations	6.25, 12.5, 25 μM
	Incubation Time	24 h
	Method	The cell viability of RAW264.7 cells, peritoneal macrophages, and HEK293 cells during TQ exposure for 24 h is determined by a conventional MTT assay.

In Vivo
In vivo	Treatment of LPS/D-GalN–induced hepatitis and EtOH/HCl–induced gastritis mouse models with TQ significantly ameliorates disease symptoms^[1].
Animal Research	Animal Models	an HCl/EtOH-induced gastritis mouse model (Male C57BL/6 and ICR mice)
	Dosages	5 and 25 mg/kg
	Administration	Oral administration

References

Chemical Information & Solubility

Molecular Weight	164.20	Formula	C₁₀H₁₂O₂
CAS No.	490-91-5	SDF	Download Thymoquinone SDF
Smiles	CC1=CC(=O)C(=CC1=O)C(C)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 32 mg/mL ( (194.88 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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