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ATP

Synonyms: Adenosine-Triphosphate, Adenosine 5'-triphosphate

ATP (Adenosine-Triphosphate, Adenosine 5'-triphosphate) is a multifunctional nucleoside triphosphate and an important endogenous signaling molecule in immunity and inflammation.

ATP Chemical Structure

ATP Chemical Structure

CAS No. 56-65-5

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ATP Related Products

Biological Activity

Description ATP (Adenosine-Triphosphate, Adenosine 5'-triphosphate) is a multifunctional nucleoside triphosphate and an important endogenous signaling molecule in immunity and inflammation.
In vitro
In vitro

ATP, a multifunctional nucleoside triphosphate and an important endogenous signaling molecule, restores signaling responses to anti-TCR treatment in Jurkat cells.[2]
Cell Research Cell lines Jurkat cells
Concentrations 50 mM
Incubation Time --
Method

Cells are washed with calcium-free and magnesium-free 1×DPBS to remove calcium and magnesium. Then cells are suspended with 450 µL 1×DPBS into a 1.5 mL centrifugal tube, treated with 50 mL Streptolysin O (0.5 µg/ml), and incubated 60 mins at 37℃. Next, cells are centrifuged at 400 g for 4 mins to discard the supernatant. Cells are re-suspended with 100 mL 1×DPBS, treated with 11 µL 50 mM ATP, and incubated in the 37℃ for indicated time.
In Vivo
In vivo

ATP can activate the NLRP3 inflammasome and enhance the production of pro-inflammatory cytokines, thus protecting mice against bacterial infection.[3]
Animal Research Animal Models Kunming mice
Dosages 50 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04760860 Not yet recruiting
Dementia With Lewy Bodies
Qiang Zhang|University of Iowa
 October 2024 Phase 1|Phase 2
NCT05590195 Not yet recruiting
Bacterial Vaginosis|Bacterial Infections|Bacterial Vaginosis | Vaginal | Microbiology|Vaginal Infection
Jeremy Burton|Deerland Enzymes|Lawson Health Research Institute|St. Joseph''s Health Care London
 May 1 2024 Phase 3
NCT06167512 Not yet recruiting
Multi-organ Failure After Severe Trauma
Assistance Publique - Hôpitaux de Paris
 April 1 2024 --

Chemical Information & Solubility

Molecular Weight 507.18 Formula

C10H16N5O13P3
CAS No. 56-65-5 SDF --
Smiles NC1=C2N=C[N](C3OC(CO[P](O)(=O)O[P](O)(=O)O[P](O)(O)=O)C(O)C3O)C2=NC=N1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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