Home Immunology & Inflammation Immunology & Inflammation related chemical Scopoletin For research use only.

Scopoletin

Synonyms: Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid

Scopoletin (Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid) is the main bioactive constituent of flower buds from Magnolia fargesii with anti-inflammatory, hypouricemic, and antioxidant activities.

Scopoletin Chemical Structure

Scopoletin Chemical Structure

CAS No. 92-61-5

Purity & Quality Control

Scopoletin Related Products

Biological Activity

Description Scopoletin (Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid) is the main bioactive constituent of flower buds from Magnolia fargesii with anti-inflammatory, hypouricemic, and antioxidant activities.
In vitro
In vitro Scopoletin (1-50 μg/ml) inhibits the release of PGE2, TNF-a, IL-1b and IL-6 and suppresses the expression of COX-2 in a concentration-dependent manner[1]. In vitro studies reveal that scopoletin lessens rotenone-induced apoptosis in SH-SY5Y cells through preventing oxidative injury. Particularly, scopoletin markedly up-regulates DJ-1, which then promotes the nuclear translocation of Nrf2 and transactivation of antioxidant genes. Scopoletin prevents the nuclear exportation of Nrf2 by reducing the levels of Keap1 and thereby enhancing the neuronal defence system[3].
Cell Research Cell lines RAW 264.7 cell line
Concentrations 1-50 μg/ml
Incubation Time 2 h
Method Cells (1×106/ml) are pre-incubated 2 h with scopoletin (1-50 μg/ml) and further cultured 6 h or 12 h with LPS (100 ngyml) in 24-well plates. Supernatants are removed at the allotted times and PGE2, TNF-a, IL-1b and IL-6 levels are quantified by immunoassay kits.
In Vivo
In vivo Scopoletin expressively represses the aortic oxido-lipidemic stress and thereby upholding normal morphology of the aorta, and thus minimizing the peril of CVD[2].
Animal Research Animal Models Healthy male albino rats of Wistar strain
Dosages 10 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 192.17 Formula

C10H8O4
CAS No. 92-61-5 SDF Download Scopoletin SDF
Smiles COC1=C(C=C2C(=C1)C=CC(=O)O2)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 38 mg/mL ( (197.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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