Scopoletin

Catalog No.S3881 Batch:S388102

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Technical Data

Formula

C10H8O4
Molecular Weight 192.17 CAS No. 92-61-5
Solubility (25°C)* In vitro DMSO 38 mg/mL (197.74 mM)
Ethanol 10 mg/mL (52.03 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Scopoletin (Esculetin 6-methyl ether, Gelseminic acid, 6-Methylesculetin, Chrysatropic acid) is the main bioactive constituent of flower buds from Magnolia fargesii with anti-inflammatory, hypouricemic, and antioxidant activities.
In vitro Scopoletin (1-50 μg/ml) inhibits the release of PGE2, TNF-a, IL-1b and IL-6 and suppresses the expression of COX-2 in a concentration-dependent manner[1]. In vitro studies reveal that scopoletin lessens rotenone-induced apoptosis in SH-SY5Y cells through preventing oxidative injury. Particularly, scopoletin markedly up-regulates DJ-1, which then promotes the nuclear translocation of Nrf2 and transactivation of antioxidant genes. Scopoletin prevents the nuclear exportation of Nrf2 by reducing the levels of Keap1 and thereby enhancing the neuronal defence system[3].
In vivo Scopoletin expressively represses the aortic oxido-lipidemic stress and thereby upholding normal morphology of the aorta, and thus minimizing the peril of CVD[2].

Protocol (from reference)

Cell Assay:[2]
  • Cell lines

    RAW 264.7 cell line

  • Concentrations

    1-50 μg/ml

  • Incubation Time

    2 h

  • Method

    Cells (1×106/ml) are pre-incubated 2 h with scopoletin (1-50 μg/ml) and further cultured 6 h or 12 h with LPS (100 ngyml) in 24-well plates. Supernatants are removed at the allotted times and PGE2, TNF-a, IL-1b and IL-6 levels are quantified by immunoassay kits.

Animal Study:[2]
  • Animal Models

    Healthy male albino rats of Wistar strain

  • Dosages

    10 mg/kg

  • Administration

    oral

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.