Home Immunology & Inflammation Immunology & Inflammation related chemical Tetramethylpyrazine For research use only.

Tetramethylpyrazine

Synonyms: ligustrazine

Tetramethylpyrazine (ligustrazine, TMP) is a natural compound isolated from Chinese herbal medicine Ligusticum wallichii (Chuan Xiong) with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.

Tetramethylpyrazine Chemical Structure

Tetramethylpyrazine Chemical Structure

CAS No. 1124-11-4

Purity & Quality Control

Batch: S395601 Ethanol]100 mg/mL]false]DMSO]27 mg/mL]false]Water]8 mg/mL]true Purity: 99.96%
99.96

Tetramethylpyrazine Related Products

Biological Activity

Description Tetramethylpyrazine (ligustrazine, TMP) is a natural compound isolated from Chinese herbal medicine Ligusticum wallichii (Chuan Xiong) with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.
Targets
PDE [3]
In vitro
In vitro Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP could restrain mitochondrial ROS generation and upregulate the expression of PGC1, NRF1, and Tfam, which reflects mitochondrial biogenesis. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can exert antiapoptosis ability by inhibiting macrophage COX-2. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed[1].
Cell Research Cell lines HL-60 cells
Concentrations 300 µg/mL
Incubation Time 24, 48, 60, 72 h
Method

Cell viability is determined by the MTT assay. HL-60 cells are treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively.
In Vivo
In vivo Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation are significantly upregulated after Tetramethylpyrazine (TMP) pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. TMP is reported to possess a broad spectrum of pharmacological effects, such as antioxidant, anti-inflammatory, antifibrosis effects. Early pharmacokinetic research has determined the metabolism rate of TMP and verified its in-vivo short half-life of T1/2=2.89 h[1]. TMP is found to protect ischemic brain damage, and promote cell proliferation and differentiation stimulated by ischemia[2].
Animal Research Animal Models A rat model of Parkinson's disease induced by MPTP (Wistar rats)
Dosages 20 mg/kg/d
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06122701 Not yet recruiting
Healthy
University Medicine Greifswald
 November 20 2023 Not Applicable
NCT06025396 Completed
Cocaine Use Disorder|Substance Use Disorders|Healthy Volunteers
Tempero Bio Inc.|National Institute on Drug Abuse (NIDA)
 January 6 2023 Phase 1
NCT05509595 Active not recruiting
Fibrous Dysplasia Of Bone
National Institute of Dental and Craniofacial Research (NIDCR)|National Institutes of Health Clinical Center (CC)
 December 7 2022 Phase 2
NCT05357573 Active not recruiting
Tumor-Induced Osteomalacia (TIO)
Kyowa Kirin Co. Ltd.
 September 7 2022 Phase 4

Chemical Information & Solubility

Molecular Weight 136.19 Formula

C8H12N2
CAS No. 1124-11-4 SDF Download Tetramethylpyrazine SDF
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 100 mg/mL

DMSO : 27 mg/mL ( (198.25 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 8 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Tetramethylpyrazine | Tetramethylpyrazine supplier | purchase Tetramethylpyrazine | Tetramethylpyrazine cost | Tetramethylpyrazine manufacturer | order Tetramethylpyrazine | Tetramethylpyrazine distributor