Home Immunology & Inflammation Immunology & Inflammation related chemical Sophoricoside For research use only.

Sophoricoside

Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae) with numerous pharmacological effects, including anti- inflammatory, anti-cancer and immunosuppressive effects.

Sophoricoside Chemical Structure

Sophoricoside Chemical Structure

CAS No. 152-95-4

Purity & Quality Control

Batch: S377601 DMSO]86 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.90%
99.90

Sophoricoside Related Products

Biological Activity

Description Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae) with numerous pharmacological effects, including anti- inflammatory, anti-cancer and immunosuppressive effects.
In vitro
In vitro Treatment with sophoricoside at concentrations of 1–10 μM inhibited lipid accumulation in HepG2 cells in a dose-dependent manner. At the same concentration range, no effect on cell viability was observed in the MTT assay. Inhibition of lipogenesis was associated with the downregulation of SREBP-1a, SREBP-1c, SREBP-2 and their downstream target genes (FAS, ACC, HMGR). Sophoricoside has the capability to increase glucose uptake by C2C12 myotubes. It also effectively inhibited the activities of α-glucosidase and α-amylase in vitro[1].
Cell Research Cell lines HepG2 Cells
Concentrations 0-75 μM
Incubation Time 48 h
Method

HepG2 cells were cultured in a 24-well plate. After reaching confluence, the cells were incubated for 48 h in the presence of sophoricoside. Subsequently, the culture medium was removed and replaced by 500 μL of fresh culture medium containing 10% sterile filtered MTT. After 3 h, the insoluble formazan crystals were dissolved in 500 μL/well isopropanol and absorbance was measured at 570 nm, using the 630 nm reading as a reference.
In Vivo
In vivo Sophoricoside remarkably lowered postprandial hyperglycaemia in starch-loaded C57BL6/J mice[1].
Animal Research Animal Models C57BL6/J mice
Dosages 50, 100 and 200 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 432.38 Formula

C21H20O10
CAS No. 152-95-4 SDF --
Smiles C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)OC4C(C(C(C(O4)CO)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 86 mg/mL ( (198.89 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Sophoricoside | Sophoricoside supplier | purchase Sophoricoside | Sophoricoside cost | Sophoricoside manufacturer | order Sophoricoside | Sophoricoside distributor