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Ganoderic acid A Immunology & Inflammation related inhibitor

Name: Ganoderic acid A
Brand: Selleck Chemicals
SKU: S4753
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4753

Ganoderic Acid A (GAA) is an active triangle compound in Ganoderma Lucidum. It can inhibit the JAK-STAT3 signal pathway and inhibit cell proliferation, vitality, and ROS. Ganoderic Acid A has analgesic, antioxidant, cytotoxicity, protecting liver and anti -tumor activity.

Chemical Structure

Molecular Weight: 516.67

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S475301 DMSO]100 mg/mL]false]Ethanol]100 mg/mL]false]Water]Insoluble]false Purity: 99.78%

99.78

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description
Bel7402 cells	Cytotoxicity assay		Cytotoxicity against human Bel7402 cells, IC50=7.25 μM
P388 cells	Cytotoxicity assay		Cytotoxicity against mouse P388 cells, IC50=7.25 μM
SGC7901 cells	Cytotoxicity assay		Cytotoxicity against human SGC7901 cells, IC50=7.25 μM
HepG2 cells	Function assay	48 h	Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay, IC50=38.7 μM
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	516.67	Formula	C₃₀H₄₄O₇	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	81907-62-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(CC(=O)CC(C)C(=O)O)C1CC(C2(C1(CC(=O)C3=C2C(CC4C3(CCC(=O)C4(C)C)C)O)C)C)O

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (193.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	GAA exhibits antitumor activity on human osteosarcoma, lymphoma, meningioma and breast cancer cells through suppressing growth and invasive behavior and/or inducing apoptosis of cancer cells. GAA could also enhance chemosensitivity of HepG2 cells to Cisplatin^[1]. GA-A treatment induces caspase-dependent apoptotic cell death characterized by a dose-dependent increase in active caspases 9 and 3, up-regulation of pro-apoptotic BIM and BAX proteins, and a subsequent loss of mitochondrial membrane potential with release of cytochrome c. Lower doses of GA-A enhance HLA class II-mediated antigen presentation and CD4+ T cell recognition of lymphoma in vitro^[2].
In vivo	GAA can significantly prolong the survival of EL4 syngeneic mice and decrease tumor metastasis to the liver, and enhance cell-mediated immune responses by attenuating myeloid-derived suppressor cells. GAA could undergo extensive metabolism, including reduction, oxidation, and hydroxylation phase I metabolism, and glucuronidation and sulfation phase II metabolism^[1].
References	[1] https://pubmed.ncbi.nlm.nih.gov/28326038/ [2] https://pubmed.ncbi.nlm.nih.gov/25142864/

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