Home Epigenetics Sirtuin activator Fisetin For research use only.

Fisetin

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Fisetin Chemical Structure

Fisetin Chemical Structure

CAS No. 528-48-3

Purity & Quality Control

Fisetin Related Products

Signaling Pathway

Sirtuin Signaling Pathways

Sirtuin Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-453 Cell viability assay 0, 20, 40, 60, 80, and 100 µM 24, 48, and 72 hours With increasing concentrations of fisetin (0, 20, 40, 60, 80, and 100 µM), there was a decrease observed in cell number in different time intervals (24, 48, and 72 hours). 30431692
SGC7901 Proliferation assay 0, 1, 5, 10, 15 or 20 µM 48 h inhibits proliferation 29805580
A549 Cell viability assay 0, 10, 20, 30 or 40 µM 24, 48 or 72 h fisetin was effective in inhibiting the growth of A549 cells in a dose- and time-dependent manner 29467859
ARPE-19 Cell migration assay 5 or 10 μM 24 h pretreatment with fisetin markedly reduced cell migration in EGF-treated ARPE-19 cells 29296070
SCC-23 Cell viability assay 0-100 μM 24 h fisetin significantly inhibited cell viability in a dose-dependent manner (significantly when the concentration is higher than 20 μM) 29312520
Tca-8113 Cell viability assay 0-100 μM 24 h fisetin significantly inhibited cell viability in a dose-dependent manner (significantly when the concentration is higher than 20 μM) 29312520
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Biological Activity

Description Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.
Targets
Sirtuin [1]
In vitro
In vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.[2]
Cell Research Cell lines Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells
Concentrations 5, 10, or 20 μM
Incubation Time 48 h
Method

Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed. 
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-IKKα/β / IKKβ / IKKα / p-IκBα / IκBα / p-p65 / p65 XBP1s / ATF6 / CHOP / ATF4 / PERK DR3 / pNF-κB / NF-κB p-mTOR / mTOR / Raptor / Rictor 23861901
Immunofluorescence p-4EBP1 / p-eIF4B / p-rpS6 uPA 20530556
Growth inhibition assay Cell viability 19670328
In Vivo
In vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.[2]
Animal Research Animal Models Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice
Dosages 100 mg/kg
Administration p.o.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05758246 Recruiting
Sepsis|Acute Infection|Organ Failure
University of Minnesota
 August 23 2023 Phase 2

Chemical Information & Solubility

Molecular Weight 286.24 Formula

C15H10O6
CAS No. 528-48-3 SDF Download Fisetin SDF
Smiles C1=CC(=C(C=C1C2=C(C(=O)C3=C(O2)C=C(C=C3)O)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 57 mg/mL ( (199.13 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 9 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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