Fisetin

Catalog No.S2298 Batch:S229808

Technical Data

Formula	C₁₅H₁₀O₆
Molecular Weight	286.24	CAS No.	528-48-3
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (199.13 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Targets

Sirtuin ^[1]

In vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.^[2]

In vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells Concentrations 5, 10, or 20 μM Incubation Time 48 h Method Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.
Animal Study:^{^[2]}	Animal Models Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice Dosages 100 mg/kg Administration p.o.

Cell Assay:^{^[2]}

Cell lines
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells
Concentrations
5, 10, or 20 μM
Incubation Time
48 h
Method
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.

Animal Study:^{^[2]}

Animal Models
Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice
Dosages
100 mg/kg
Administration
p.o.

References

Customer Product Validation

: Data from [Data independently produced by , , Alcohol Clin Exp Res, 2016, 40(10):2076-2084.]

Selleck's Fisetin has been cited by 43 publications

Pharmacological targeting of P300/CBP reveals EWS::FLI1-mediated senescence evasion in Ewing sarcoma [ Mol Cancer, 2024, 23(1):222]	PubMed: 39367409
TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer [ Cell Death Dis, 2024, 15(8):608]	PubMed: 39168971
SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure [ PLoS Pathog, 2024, 20(8):e1012291]	PubMed: 39102426
Senolytic intervention improves cognition, metabolism, and adiposity in female APPNL-F/NL-F mice [ Geroscience, 2024, 10.1007/s11357-024-01308-8]	PubMed: 39120687
Single-cell morphology encodes functional subtypes of senescence in aging human dermal fibroblasts [ bioRxiv, 2024, 2024.05.10.593637]	PubMed: 38798365
Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 [ Nat Commun, 2023, 14(1):4033]	PubMed: 37468473
Fisetin ameliorates fibrotic kidney disease in mice via inhibiting ACSL4-mediated tubular ferroptosis [ Acta Pharmacol Sin, 2023, 10.1038/s41401-023-01156-w]	PubMed: 37696989
Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity [ JCI Insight, 2023, 8(8)e166643]	PubMed: 36917180
Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties [ Cell Biosci, 2023, 13(1):176]	PubMed: 37743465
PPARγ activation by fisetin mitigates vascular smooth muscle cell senescence via the mTORC2-FoxO3a-autophagy signaling pathway [ Biochem Pharmacol, 2023, S0006-2952(23)00485-9]	PubMed: 37890594

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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