Home Epigenetics Sirtuin inhibitor - HBV inhibitor OSS_128167 For research use only.

OSS_128167

Synonyms: SIRT6-IN-1

OSS_128167 (SIRT6-IN-1) is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively. OSS_128167 has anti-viral effect in HBV transcription and replication.

OSS_128167 Chemical Structure

OSS_128167 Chemical Structure

CAS No. 887686-02-4

Purity & Quality Control

OSS_128167 Related Products

Signaling Pathway

Sirtuin Signaling Pathways

Sirtuin Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 Function assay 100 uM 18 hrs Inhibition of SIRT6 in human BxPC3 cells assessed as increase in H3K9 acetylation at 100 uM after 18 hrs by immunoblot analysis 24785705
BxPC3 Function assay 12.5 to 200 uM 24 hrs Increase in GLUT1 expression in human BxPC3 cells at 12.5 to 200 uM after 24 hrs by Western blotting analysis 24785705
Click to View More Cell Line Experimental Data

Biological Activity

Description OSS_128167 (SIRT6-IN-1) is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively. OSS_128167 has anti-viral effect in HBV transcription and replication.
Targets
SIRT6 [1]
(Cell-free)		 SIRT2 [1]
(Cell-free)		 SIRT1 [1]
(Cell-free)
89 μM 751 μM 1578 μM
In vitro
In vitro OSS-128167 is a selective compounds toward SIRT6 with IC50 values toward SIRT1 and SIRT2 being approximately 20 times higher. It is cell permeable and active in cultured cells. OSS-128167 increases H3K9 acetylation and GLUT-1 expression[1].
Cell Research Cell lines BxPC3 cells
Concentrations 100 μM
Incubation Time 0, 2, 6, 18, 24 h
Method

4 × 105 BxPC3 cells are plated in six-well plates and allowed to adhere for 24 h. Thereafter, cells are stimulated with 100 μM OSS-128167 or the respective amounts of vehicle DMSO for the indicated time amounts. Finally, cells are used for protein lysate generation, and total H3 and acetylated H3K9 levels are detected by immunoblotting.

Chemical Information & Solubility

Molecular Weight 366.32 Formula

C19H14N2O6
CAS No. 887686-02-4 SDF Download OSS_128167 SDF
Smiles C1=CC(=CC(=C1)NC(=O)C2=CC=CO2)C(=O)NC3=CC(=C(C=C3)O)C(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 73 mg/mL ( (199.27 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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