OSS_128167

Catalog No.S8627 Batch:S862701

Technical Data

Formula	C₁₉H₁₄N₂O₆
Molecular Weight	366.32	CAS No.	887686-02-4
Solubility (25°C)*	In vitro	DMSO	73 mg/mL (199.27 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

OSS_128167 (SIRT6-IN-1) is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively. OSS_128167 has anti-viral effect in HBV transcription and replication.

Targets

SIRT6 ^[1] (Cell-free)	SIRT2 ^[1] (Cell-free)	SIRT1 ^[1] (Cell-free)
89 μM	751 μM	1578 μM

In vitro

OSS-128167 is a selective compounds toward SIRT6 with IC50 values toward SIRT1 and SIRT2 being approximately 20 times higher. It is cell permeable and active in cultured cells. OSS-128167 increases H3K9 acetylation and GLUT-1 expression^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines BxPC3 cells Concentrations 100 μM Incubation Time 0, 2, 6, 18, 24 h Method 4 × 10⁵ BxPC3 cells are plated in six-well plates and allowed to adhere for 24 h. Thereafter, cells are stimulated with 100 μM OSS-128167 or the respective amounts of vehicle DMSO for the indicated time amounts. Finally, cells are used for protein lysate generation, and total H3 and acetylated H3K9 levels are detected by immunoblotting.

Cell Assay:^{^[1]}

Cell lines
BxPC3 cells
Concentrations
100 μM
Incubation Time
0, 2, 6, 18, 24 h
Method
4 × 10⁵ BxPC3 cells are plated in six-well plates and allowed to adhere for 24 h. Thereafter, cells are stimulated with 100 μM OSS-128167 or the respective amounts of vehicle DMSO for the indicated time amounts. Finally, cells are used for protein lysate generation, and total H3 and acetylated H3K9 levels are detected by immunoblotting.

References

[1] Parenti MD, et al. J Med Chem. 2014, 57(11):4796-804.

Selleck's OSS_128167 has been cited by 18 publications

Sirtuin 6 inhibits group 3 innate lymphoid cell function and gut immunity by suppressing IL-22 production [ Front Immunol, 2024, 15:1402834]	PubMed: 39253083
Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery [ Antioxidants (Basel), 2023, 12(3)714]	PubMed: 36978961
Polydatin Attenuates Cisplatin-Induced Acute Kidney Injury via SIRT6-Mediated Autophagy Activation [ Oxid Med Cell Longev, 2022, 2022:9035547]	PubMed: 36160707
SIRT6 regulates obesity-induced oxidative stress via ENDOG/SOD2 signaling in the heart [ Cell Biol Toxicol, 2022, 10.1007/s10565-022-09735-z]	PubMed: 35798905
Valdecoxib attenuates lipid-induced hepatic steatosis through autophagy-mediated suppression of endoplasmic reticulum stress [ Biochem Pharmacol, 2022, 199:115022]	PubMed: 35358477
Downregulation of Sirt6 by CD38 promotes cell senescence and aging [ Aging (Albany NY), 2022, 14(23):9730-9757]	PubMed: 36490326
Downregulation of Sirt6 by CD38 promotes cell senescence and aging [ Aging (Albany NY), 2022, 14(23):9730-9757]	PubMed: 36490326
Deacetylation of ATG4B promotes autophagy initiation under starvation [ Sci Adv, 2022, 8(31):eabo0412]	PubMed: 35921421
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1 [ NPJ Precis Oncol, 2021, 5(1):20]	PubMed: 33742100
SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation [ Front Cell Dev Biol, 2021, 9:625540]	PubMed: 33718364

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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