Home Cytoskeletal Signaling Sirtuin inhibitor 3-TYP For research use only.

3-TYP

Synonyms: 3-(1H-1,2,3-triazol-4-yl) pyridine

3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor which is selective for Sirt3 over Sirt1 and Sirt2. The IC50 values for SITR1, SIRT2, SIRT3 are 88 nM, 92 nM, 16 nM respectively.

3-TYP Chemical Structure

3-TYP Chemical Structure

CAS No. 120241-79-4

Purity & Quality Control

3-TYP Related Products

Signaling Pathway

Sirtuin Signaling Pathways

Sirtuin Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa Cytotoxicity assay 1 to 6 hrs Cytotoxicity against human HeLa cells assessed as cell death after 1 to 6 hrs, IC50=4.7μM 22835719
SK-MEL-28 Function assay 100 uM 24 hrs Reduction in ATP level in human SK-MEL-28 cells at 100 uM maintained in Locke's solution after 24 hrs by luciferase-based assay in absence of GF and glucose 22835719
SK-MEL-28 Function assay 100 uM 24 hrs Increase in superoxide level in human SK-MEL-28 cells maintained in medium containing GF and glucose at 100 uM after 24 hrs by DHE-based fluorescence ssay 22835719
SK-MEL-28 Function assay 100 uM 24 hrs Increase in superoxide level in human SK-MEL-28 cells at 100 uM maintained in Locke's solution after 24 hrs by DHE-based fluorescence assay in absence of GF and glucose 22835719
Click to View More Cell Line Experimental Data

Biological Activity

Description 3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor which is selective for Sirt3 over Sirt1 and Sirt2. The IC50 values for SITR1, SIRT2, SIRT3 are 88 nM, 92 nM, 16 nM respectively.
Targets
SIRT3 [1]
(cell-free)		 SIRT1 [1]
(cell-free)		 SIRT2 [1]
(cell-free)
16 nM 88 nM 92 nM
In vitro
In vitro Melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity are significantly attenuated by 3-TYP in HepG2 cells exposed to Cd (cadmium)[2].
Cell Research Cell lines HepG2 cells
Concentrations 50 μM
Incubation Time 12 h
Method

Cells are randomly divided into the following treatment groups: 10 μM Cd; melatonin (1 μM)+10 μM Cd; 3-TYP (50 μM)+10 μM Cd; and 3-TYP (50 μM)+melatonin (1 μM)+10 μM Cd. cells are treated for 12 h. The role of the SIRT3-SOD2 pathway in hepatic cell protection after melatonin pretreatment is investigated.

Chemical Information & Solubility

Molecular Weight 146.15 Formula

C7H6N4
CAS No. 120241-79-4 SDF Download 3-TYP SDF
Smiles C1=CC(=CN=C1)C2=NNN=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 29 mg/mL ( (198.42 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 29 mg/mL

Water : 1.25 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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